The Ceralpha method is routinely run at 40˚C, but it can be used at temperatures up to 60˚C. If it is run at higher temperatures (up to 60˚C) then higher activity values will be obtained. This assumes that the enzymes being analysed (alpha-amylase) are stable at these higher temperatures. If you need to measure activity at temperatures above 60˚C you can use the Megazyme Amylazyme tablets or Red Starch.
Can the α-Amylase Assay Kit (Ceralpha Method) (K-CERA) reagent be used at high temperatures?
Modified on: Wed, 10 Nov, 2021 at 3:54 PM
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