The Ceralpha Alpha-Amylase reagent (R-CAAR4) can only be used in the pH range 5.0-6.0, as stated in the data sheet. To assay amylase at pH 6-7 you will need the Amylase HR reagent (R-AMHR4) which contains a thermostable α-glucosidase and allows assays to be performed at temperatures up to 60°C and over the pH range 5.2-7.5. This reagent is sold separately (R-AMHR4) and is also a component in the Ceralpha Kit (K-CERA).
Is the Ceralpha Alpha-Amylase Assay Reagent (R-CAAR4) or the Amylase HR Assay Reagent (R-AMHR4) preferable for use a pH 6-7?
Modified on: Wed, 30 Mar, 2016 at 10:42 AM
Did you find it helpful?Send feedback
Sorry we couldn't be helpful. Help us improve this article with your feedback.