D-Glucose (GOPOD Format) (K-GLUC)

Is it possible to read absorbances for GOPOD Reagent reaction used in the D-Glucose Assay Kit (K-GLUC) at a wavelength other than 510 nm?
While we recommend reading the absorbance at 510 nm you can use different wavelength if that is your preference. However please note this may give a large...
Wed, 20 Apr, 2016 at 4:46 PM
Megazyme supplies different kits that use GOPOD Reagent. Is GOPOD Reagent interchangeable between these kits, e.g.: can GOPOD Reagent from Total Starch kit be used in Resistant Starch procedure?
The GOPOD Reagent Buffer and Enzymes are the same in all Megazyme kits and can be used interchangeably. Product Page (K-GLUC)
Wed, 20 Apr, 2016 at 4:37 PM
Is the colour developed with GOPOD Reagent in the D-Glucose Assay Kit (K-GLUC) stable at room temperature? Does the reaction continue after 20 minutes of incubation in the water bath at 40°C?
This is an end-point assay. The reaction is complete after approximately 15 min, however we recommend incubating the samples for 20 min to ensure that the...
Wed, 20 Apr, 2016 at 4:46 PM
Should GOPOD Reagent (K-GLUC) be a clear, colourless solution after preparation?
The GOPOD Regent may be light yellow or light pink in colour when freshly prepared. It will develop a stronger pink colour over 2-3 months at 4°C. The col...
Wed, 20 Apr, 2016 at 4:37 PM
Is it possible to check if the prepared GOPOD Reagent (K-GLUC) is still stable after storage?
The GOPOD Reagent is still usable when the absorbance of this solution is less than 0.05 when read against distilled water. Product Page (K-GLUC)
Wed, 20 Apr, 2016 at 4:38 PM
Can I make less than 1 L of GOPOD Reagent at once?
It is not possible to prepare less than 1 L of the GOPOD Reagent at once, the components should not be split into smaller volumes. Bottle 4 contains freez...
Wed, 20 Apr, 2016 at 4:38 PM
Should the pH of the sample be adjusted when being analysed using the D-Glucose Assay Kit (GOPOD Format) (K-GLUC)? At what pH do glucose oxidase and peroxidase work?
The test is best run at pH 7.4. The reagent is buffered at this pH. Using different pH values may affect results, i.e. it may take longer to reach the end...
Wed, 20 Apr, 2016 at 4:42 PM
Is the glucose colour production in the D-Glucose Assay Kit (GOPOD Format) (K-GLUC) affected by any components likely to be present in samples? Is it possible to check this?
The colour production may be affected by high salt concentrations above 0.2 M and possibly by ascorbate. To test for possible effects on colour developmen...
Wed, 20 Apr, 2016 at 4:47 PM
Is it necessary to run a glucose standard with each set of determination using the D-Glucose Assay Kit (GOPOD Format) (K-GLUC)?
It is necessary to incubate glucose standards (recommended in quadruplicate) with each set of determinations. Firstly, the intensity of the colour respon...
Wed, 20 Apr, 2016 at 4:36 PM
What light path do I need to use to measure the absorbance of GOPOD Reagent used in the D-Glucose Assay Kit (K-GLUC)?
The absorbance of 100 micrograms of glucose (0.1mL of standard at 1.0 mg/mL) incubated with 3 mL of GOPOD Reagent measured at 510 nm and with a light path ...
Wed, 20 Apr, 2016 at 4:48 PM