β-Glucan Assay Kit (Mixed Linkage) (K-BGLU)

Is it necessary to add sodium azide to the β-Glucan Assay Kit (K-BGLU) buffers that are not supplied with the kit (sodium phosphate and sodium acetate)?
The sodium azide is a preservative preventing the microbial growth in the buffer. It can be excluded from the buffer without affecting the performance of ...
Fri, 22 Apr, 2016 at 9:48 AM
Is there any tolerance for the pH of buffers used in the β-Glucan Assay Kit (K-BGLU)?
We highly recommend using buffer at pH stated in the booklet. Usually with enzymes, you will get up to 80% of maximal activity with 0.5 pH units of the op...
Fri, 22 Apr, 2016 at 9:48 AM
Is it necessary to use sodium dihydrogen orthophosphate to make 20 mM sodium phosphate buffer for use with the Mixed-Linkage β-Glucan Assay Kit (K-BGLU)?
Any sodium phosphate can be used, once the pH is adjusted to pH 6.5. Product Page (K-BGLU)
Fri, 22 Apr, 2016 at 9:49 AM
Is it possible to read absorbances for GOPOD Reagent reaction used in the β-Glucan Assay Kit (Mixed Linkage) (K-BGLU) at a wavelength other than 510 nm?
While we recommend reading the absorbance at 510 nm you can use different wavelength if that is your preference. However please note this may give a large...
Fri, 22 Apr, 2016 at 9:50 AM
Megazyme supplies different kits that use GOPOD Reagent. Is GOPOD Reagent interchangeable between these kits, e.g.: can GOPOD Reagent from Total Starch kit be used in β-Glucan Assay Kit (Mixed Linkage) (K-BGLU)?
The GOPOD Reagent Buffer and Enzymes are the same in all Megazyme kits and can be used interchangeably. Product Page (K-BGLU)
Fri, 22 Apr, 2016 at 9:50 AM
Is the colour developed with GOPOD Reagent from β-Glucan Assay Kit (Mixed Linkage) (K-BGLU) stable at room temperature? Does the reaction continue after 20 minutes of incubation in the water bath at 40°C?
This is an end-point assay. The reaction is complete after approximately 15 min, however we recommend incubating the samples for 20 min to ensure that the...
Fri, 22 Apr, 2016 at 9:51 AM
Should GOPOD Reagent from β-Glucan Assay Kit (Mixed Linkage) (K-BGLU) be a clear, colourless solution after preparation?
The GOPOD Regent may be light yellow or light pink in colour when freshly prepared. It will develop a stronger pink colour over 2-3 months at 4°C. The col...
Fri, 22 Apr, 2016 at 9:51 AM
Can I make less than 1 L of GOPOD Reagent at once for use with the β-Glucan Assay Kit (Mixed Linkage) (K-BGLU)?
It is not possible to prepare less than 1 L of the GOPOD Reagent at once, the components should not be split into smaller volumes. Bottle 4 contains freez...
Fri, 22 Apr, 2016 at 9:52 AM
Is it necessary to run a glucose standard with each set of determinations (incubation with GOPOD Reagent) with β-Glucan Assay Kit (Mixed Linkage) (K-BGLU)?
It is necessary to incubate glucose standards (recommended in quadruplicate) with each set of determinations. Firstly, the intensity of the colour respon...
Fri, 22 Apr, 2016 at 9:52 AM
What light path do I need to use to measure the absorbance of GOPOD Reagent while using β-Glucan Assay Kit (K-BGLU)?
The absorbance of 100 micrograms of glucose (0.1mL of standard at 1.0 mg/mL + 0.1 mL sodium acetate buffer) incubated with 3 mL of GOPOD Reagent measured a...
Fri, 22 Apr, 2016 at 9:46 AM