You can run the Cellazyme method at pH 6.2. We suggest that you use a Maleate buffer (prepared from maleic acid at 25 mM concentration). We would also suggest that you stop the reaction with tri-sodium phosphate (adjusted to pH 11.0). This will however affect the standard curve as different parameters are being used. A new standard curve can be generated using your own conditions by standardising the activity of a purified -cellulase on say CM-Cellulose 4M with Nelson/Somogyi reducing sugar method. Then, use this standardised enzyme to produce your standard curve. This is the way we produce our standard curves.
Modified on: Tue, 22 Mar, 2016 at 10:38 AM
Did you find it helpful?Send feedback
Sorry we couldn't be helpful. Help us improve this article with your feedback.