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Acetaldehyde (K-ACHYD) Assay Protocol
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Acetic Acid (ACS Manual Format) (K-ACET) Assay Protocol
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Acetic Acid (ACS Analyser Format) (K-ACETAF) Assay Protocol
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Acetyl-CoA synthetase (Bacillus subtilis) (E-ACSBS) Data Sheet
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endo-α-N-Acetylgalactosaminidase (Enterococcus faecalis) (E-OGLYEF) Data Sheet
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α-N-Acetylgalactosaminidase (microbial) (E-ANAGM) Data Sheet
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Blocked 4-nitrophenyl-α-maltoheptaoside (O-BPNPC7) Data Sheet
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β-Naphthyl-β-maltotrioside (O-NAPC3-100) Data Sheet
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4-Nitrophenyl-β-maltotrioside (O-PNPC3-100) Data Sheet
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Amylazyme Tablets (T-AMZ) Assay Protocol
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Amylazyme BG Tablets (T-AMZBG-200T) Assay Protocol
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Amylazyme Red Tablets (T-AMZRD) Assay Protocol
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AZCL-Amylose (I-AZAMY) Assay Protocol
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AZCL-Amylose (fine) (I-AZAMYF) Assay Protocol
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RedCL-Amylose (fine) (I-RCLAMYF) Assay Protocol
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Red Debranched Arabinan (Sugar Beet) (S-RDAR) Assay Protocol
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Red Starch (S-RSTAR) Assay Protocol
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Azo-CM-Cellulose (Liquid) (S-ACMCL) Assay Protocol
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Amylose (potato) (P-AMYL) Data Sheet
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Arabinan (Sugar Beet) (P-ARAB) Data Sheet
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Debranched Arabinan (Sugar Beet) (P-DBAR) Data Sheet
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MegaQuant™ Wave Spectrophotometer (Chemistry Analyzer) (D-MQWAVE) Data Booklet
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MegaQuant Colorimeter Tubes (D-MQTUB) Data Sheet
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Megazyme Incubation Bath MK III (D-IBMKIII) Data Sheet
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Are the enzymes that are used in the Megazyme products stable when shipped at ambient temperature?
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Should the pH of the sample be adjusted even for samples in acidic media?
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I have some doubts about the appearance/quality of a kit component what should be done?
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When using the K-ACHYD Assay Kit for quantitative analysis what level of accuracy and repeatability can be expected?
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What is the detection limit of the K-ACHYD Assay Kit?
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What is the linear range of the K-ACHYD Assay Kit?
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Does the decolourising preparation in the Acetic Acid kits remove some VA during the process?
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What are the major differences between the various Acetic Acid test kits?
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Which Acetic Acid kit is recommended for a 96-well microplate format?
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When using the Acetic Acid Kit (K-ACET) for quantitative analysis what level of accuracy and repeatability can be expected?
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Is the Acetic Acid Kit (K-ACET) specific for acetate?
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What is the range of the Acetic Acid Kit (K-ACET)?
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Is the Acetic Acid Kit (K-ACETRM) specific for acetate?
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When using the Acetic Acid Kit (K-ACETRM) for quantitative analysis what level of accuracy and repeatability can be expected?
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What is the range of the Acetic Acid Kit (K-ACETRM)?
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When using the Enzymatic Yeast β-Glucan Assay Kit (K-EBHLG), should the sample pH be adjusted even for samples in acidic media?
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There are crystals in my GOPOD reagent buffer. Is this normal and will it affect my assay?
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What is difference between β-Glucan (Yeast & Mushroom) Assay Kit (K-YBGL) and Yeast Beta-Glucan Assay Kit (K-EBHLG)?
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Is the α-Amylase Assay Kit (Ceralpha Method) (K-CERA) suitable for measuring porcine pancreatic alpha-amylase?
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We would like to know where the coefficient of 18.1 (EmM of p-nitrophenol) comes from? What does it mean?
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Any recommendations on using your α-Amylase Assay Kit (Ceralpha Method) (K-CERA) assay at a low pH (pH 3-4)?
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Is either α-Amylase Assay Kit (K-CERA or K-AMYLSD) suitable for quantifying trace amounts of alpha-amylase activity in a pullulanase preparation?
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Is the α-Amylase SD Assay Kit (K-AMYLSD) suitable to measure alpha-amylase in cooked breads? If so, do I need to alter the procedure?
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How long does a single analysis take using α-Amylase SD Assay Kit (High Sensitivity Method) (K-AMYLSD) to compare with the Hagberg Falling Number method?
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Is the Ceralpha Alpha-Amylase Assay Reagent (R-CAAR4) or the Amylase HR Assay Reagent (R-AMHR4) preferable for use a pH 6-7?
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Can the Ceralpha Alpha-Amylase Assay Reagent (R-CAAR4) be used for the determination of fungal alpha-amylase?
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Can the Ceralpha Alpha-Amylase Assay Reagent (R-CAAR4) be used to analyse the alpha-amylase content of meat?
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Is the Ceralpha α-Amylase Assay Reagent (R-CAAR4) or the Amylase HR Assay Reagent (R-AMHR4) preferable for use a pH 6-7?
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Can the α-Amylase HR Assay Reagent (R-AMHR4) be used for the determination of fungal alpha-amylase?
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Any recommendations for using the α-Amylase HR Assay Reagent (R-AMHR4) at pH 3-4?
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With your Amylose/Amylopectin Assay Kit (K-AMYL) can you calculate the amylose content from the Con A supernatant absorbance without performing the total starch section if you have recorded the weight of the sample?
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Can Amylose/Amylopectin Assay Kit (K-AMYL) also be used for total starch determination?
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Do you provide any information in your Amylose/Amylopectin Kit (K-AMYL) for the separation of the two starch fractions?
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Why does the Amyloglucosidase Assay Reagent (R-AMGR3) contain PNP-beta-D-maltoside and not PNP-alpha-D-maltoside, since amyloglucosidase is specific for alpha-1,4-D-linked glucans?
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Can the Amyloglucosidase Reagent (R-AMGR3) be used to measure Amyloglucosidase (E-AMGDF) levels in malted barley and what is a suitable extraction procedure to use for ground malt?
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In what absorbance range should I use Amyloglucosidase (E-AMGDF) with Amyloglucosidase Assay Reagent (R-AMGR3)?
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When using the L-Arabinose/D-Galactose Assay Kit (K-ARGA) for quantitative analysis what level of accuracy and repeatability can be expected?
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Can oligosaccharides or polysaccharides be measured using the L-Arabinose/D-Galactose Assay Kit (K-ARGA)?
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What is the detection limit of the L-Arabinose/D-Galactose Assay Kit (K-ARGA)?
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When using the L-Arginine/Urea/Ammonia Assay Kit (K-LARGE) for quantitative analysis what level of accuracy and repeatability can be expected?
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What is the detection limit and linearity of the L-Arginine/Urea/Ammonia Assay Kit (K-LARGE)?
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My A1 values for L-Arginine/Urea/Ammonia Assay Kit (K-LARGE) blank and samples have an absorbance greater than 1.0. Is it correct for my A1 values to be this high?
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Is it possible to adapt the Ascorbic Acid Assay Kit (L-Ascorbate) (K-ASCO) for use on various auto-analysers?
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What absorbance value should be attained using the ascorbic acid standard supplied with Ascorbic Acid Assay Kit (L-Ascorbate) (K-ASCO)?
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Is it possible to measure dehydroascorbic acid with the Ascorbic Acid Assay Kit (L-Ascorbate) (K-ASCO)?
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When using the L-Asparagine/L-Glutamine/Ammonia Assay Kit (Rapid) (K-ASNAM) for quantitative analysis what level of accuracy and repeatability can be expected?
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When using the L-Asparagine/L-Glutamine/Ammonia Assay Kit (Rapid) (K-ASNAM) for quantitative analysis what level of accuracy and repeatability can be expected?
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What is the detection limit of the L-Asparagine/L-Glutamine/Ammonia Assay Kit (Rapid) (K-ASNAM)?
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Can the Cellulase Assay Kit (CellG3 Method) (K-CellG3) be used to measure thermostable cellulase enzymes?
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What assay methods do you recommend for kinetic analyses of cellulases and xylanases (kit, tablets and soluble/insoluble dyed substrates)?
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Are most cellulase more active against CellG5 reagent than the CellG3 reagent as used in the Cellulase Assay Kits (K-CellG5 or K-CellG3)?
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When using the Citric Acid Assay Kit (K-CITR) for quantitative analysis what level of accuracy and repeatability can be expected?
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What is the detection limit and linearity of the Citric Acid Assay Kit (K-CITR)?
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What is the linearity of the Citric Acid Assay Kit (K-CITR)?
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I am experiencing problems obtaining consistent/accurate results using the Ethanol Assay Kit (K-ETOH / K-ETOHLQR) – what should I do?
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When using the Ethanol Assay Kit (K-ETOH) for quantitative analysis what level of accuracy and repeatability can be expected?
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What is the range of the Ethanol Assay kit (K-ETOH)?
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What is the stability of enzymes for Total Dietary Fiber Assay Kit (K-TDFR)?
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Which kit is most suitable to determine insoluble and soluble dietary fibre in barley?
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Are the lower temperatures of 90˚C to 95˚C adequate for the hydrolysis if we extend the incubation time in the Total Dietary Fiber Assay Kit (K-TDFR) procedure?
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How to prepare the required 78% ethanol solution that is used in the Rapid Integrated Total Dietary Fiber Assay Kit (K-RINTDF)?
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Is it possible to test liquid samples with the Rapid Integrated Total Dietary Fibre Assay Kit (K-RINTDF)?
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What is the purpose of the 78% ethanol solution in the Rapid Integrated Total Dietary Fiber (K-RINTDF) Assay Procedure?
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Does the fructanase enzyme used in the Fructa Assay kits (K-FRUC & K-FRUHCK) hydrolyse Neosugar?
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When measuring fructan in starch containing samples, i.e. maize, is there a possibility of incomplete starch hydrolysis in step B of the Fructan Assay (K-FRUC) ?
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Does the Fructan Assay Kit (K-FRUC) specifically measure inulin content of a sample ?
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The calibration curve in the D-Fructose/D-Glucose Assay Kit (K-FRUGL) data booklet is based on using a 4.6 mm pathlength cuvette. Can I use 10 mm path-length cuvettes?
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Can oligosaccharides or polysaccharides be measured using the D-Fructose/D-Glucose Assay Kit (K-FRUGL)?
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When using the D-Fructose/D-Glucose Assay Kit (K-FRUGL) for quantitative analysis what level of accuracy and repeatability can be expected?
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Can oligosaccharides or polysaccharides be measured using the D-Fructose/D-Glucose Assay Kit (Liquid Ready) (K-FRGLQR)?
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When using the D-Fructose/D-Glucose Assay Kit (Liquid Ready) (K-FRGLQR) for quantitative analysis what level of accuracy and repeatability can be expected?
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What is the range of the D-Fructose/D-Glucose Assay Kit (K-FRGLQR)?
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Can oligosaccharides or polysaccharides be measured using the The D-Fructose and D-Glucose Assay Kit (MegaQuant™ Format) (K-FRGMQ)?
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When using the The D-Fructose and D-Glucose Assay Kit (MegaQuant™ Format) (K-FRGLMQ) for quantitative analysis what level of accuracy and repeatability can be expected?
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What analyser would you recommend for use with the The D-Fructose and D-Glucose Assay Kit (MegaQuant™ Format) (K-FRGLMQ)?
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Can the Galactomannan Assay Kit (K-GALM) be used to measure any type / source of galactomannan?
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Are there any differences in the assay procedures for measuring carob and guar using the Galactomannan Assay Kit (K-GALM)?
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The Galactomannan Assay Kit (K-GALM) gives the galactose:mannose ratio for carob is there any similar data published for guar gum?
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What is the Mixed Linkage β-Glucan Assay Kit (K-BGLU) used to measure? What is the specificity of this kit?
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What is mixed-linkage β -glucan that you can measure using β-Glucan Assay Kit (K-BGLU)?
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Is it possible to specifically measure cereal beta-glucan in a mixed material of mushroom/yeast and cereal/grains using the Mixed Linkage β-Glucan Assay Kit (K-BGLU)?
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What has changed with the new K-YBGL kit format?
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Why was the exo-1,3-β-Glucanase plus β-Glucosidase removed from the K-YBGL procedure for total glucan hydrolysis?
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What Matrices were used in the validation of the the β-Glucan Assay Kit (Yeast and Mushroom) (K-YBGL)?
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When using the D-Gluconic Acid Assay Kit (K-GATE) for quantitative analysis what level of accuracy and repeatability can be expected?
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What is the detection limit of the D-Gluconic Acid Assay Kit (K-GATE)?
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What is the linearity of the D-Gluconic Acid Assay Kit (K-GATE)?
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When using the D-Glucosamine Assay Kit (K-GAMINE) for quantitative analysis what level of accuracy and repeatability can be expected?
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What is the detection limit and linearity of the D-Glucosamine Assay Kit (K-GAMINE)?
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What is the linearity of the D-Glucosamine Assay Kit (K-GAMINE)?
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Will results be incorrect if it is outside a certain pH range from the pH optimum of glucose oxidase and peroxidase in the D-Glucose Assay Kit (GOPOD Format) (K-GLUC)?
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What is the linear range of the D-Glucose Assay Kit (GOPOD Format) (K-GLUC)?
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In the D-Glucose Assay Kit (GOPOD Format) (K-GLUC Assay) is the colour stable at room temperature? Does the reaction continue if not read within 20 minutes?
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How long is the GOPOD reagent (K-GLUC / R-GLC4) stable for when stored at 4˚C?
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Can the absorbance for the GOPOD assay (R-GLC4 / K-GLUC) be measured at alternative wavelengths if 510 nm is not available?
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What might be the cause of very low absorbance readings for glucose controls and samples in the GOPOD assay procedure (K-GLUC / R-GLC4)?
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Can oligosaccharides or polysaccharides be measured using the D-Glucose HK Assay Kit (K-GLUHK)?
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Can the D-Glucose Assay kit (K-GLUHK) be used to measure glucose as a component of polysaccharides in plant material?
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What is the detection limit of the D-Glucose Assay kit (K-GLUHK)?
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When using the D-Glucuronic/D-Galacturonic Acid Assay Kit (K-URONIC) for quantitative analysis what level of accuracy and repeatability can be expected?
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Can the D-Glucuronic/D-Galacturonic Acid Assay Kit (K-URONIC) be used to measure galacturonic acid in pectin?
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What is the detection limit and linearity of the D-Glucuronic/D-Galacturonic Acid Assay Kit (K-URONIC)?
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When using the L-Glutamic Acid Assay Kit (K-GLUT) for quantitative analysis what level of accuracy and repeatability can be expected?
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What is the detection limit of the L-Glutamic Acid Assay Kit (K-GLUT)?
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What is the linearity of the L-Glutamic Acid Assay Kit (K-GLUT)?
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When using the L-Glutamine/Ammonia Assay Kit (Rapid) (K-GLNAM) for quantitative analysis what level of accuracy and repeatability can be expected?
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What is the detection limit and linearity of the L-Glutamine/Ammonia Assay Kit (Rapid) (K-GLNAM)?
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What is the linearity of the L-Glutamine/Ammonia Assay Kit (Rapid) (K-GLNAM)?
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When using the Glycerol Assay Kit (K-GCROL) for quantitative analysis what level of accuracy and repeatability can be expected?
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What is the detection limit and linearity of the Glycerol Assay Kit (K-GCROL)?
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What is the linearity of the Glycerol Assay Kit (K-GCROL)?
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When using the Glycerol GK Assay Kit (K-GCROLGK) for quantitative analysis what level of accuracy and repeatability can be expected?
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What is the detection limit and linearity of the Glycerol GK Assay Kit (K-GCROLGK)?
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What is the linearity of the Glycerol GK Assay Kit (K-GCROLGK)?
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When using the D-3-Hydroxybutyric Acid (β-Hydroxybutyrate) Assay Kit (K-HDBA) for quantitative analysis what level of accuracy and repeatability can be expected?
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What is the detection limit of the D-3-Hydroxybutyric Acid (β-Hydroxybutyrate) Assay Kit (K-HDBA)?
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What is the linear range of the D-3-Hydroxybutyric (β-Hydroxybutyrate) Acid Assay Kit (K-HDBA)?
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When using the myo-Inositol Assay Kit (K-INOSL) for quantitative analysis what level of accuracy and repeatability can be expected?
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What is the detection limit of the myo-Inositol Assay Kit (K-INOSL)?
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What is the linear range of the myo-Inositol Assay Kit (K-INOSL)?
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When using the D-Isocitric Acid Assay Kit (K-ISOC) for quantitative analysis what level of accuracy and repeatability can be expected?
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What is the detection limit of the D-Isocitric Acid Assay Kit (K-ISOC)?
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What is the linear range of the D-Isocitric Acid Assay Kit (K-ISOC)?
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When using the D-Lactic Acid (Rapid) Assay Kit (K-DATE) for quantitative analysis what level of accuracy and repeatability can be expected?
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What is the detection limit and linearity of the D-Lactic Acid (Rapid) Assay Kit (K-DATE)?
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What is the linearity of the D-Lactic Acid (Rapid) Assay Kit (K-DATE)?
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When using the D-/L-Lactic Acid (Rapid) Assay Kit (K-DLATE) for quantitative analysis what level of accuracy and repeatability can be expected?
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What is the detection limit and linearity of the D-/L-Lactic Acid (Rapid) Assay Kit (K-DLATE)?
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What is the linearity of the D-/L-Lactic Acid (Rapid) Assay Kit (K-DLATE)?
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When using the L-Lactic Acid Assay Kit (K-LATE) for quantitative analysis what level of accuracy and repeatability can be expected?
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What is the detection limit and linearity of the L-Lactic Acid Assay Kit (K-LATE)?
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What is the linearity of the L-Lactic Acid Assay Kit (K-LATE)?
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Can hydrogen peroxide from other suppliers be used in this Sequential/High Sensitivity Lactose Assay Kit (K-LOLAC) method?
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Can Step C of the Sequential / High Sensitivity Lactose Assay Kit (K-LOLAC) procedure be carried out using a microplate?
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I have used Megazyme's K-LACGAR & K-LOLAC kits for determination of lactose in “lactose-free” milk and I have obtained values of 1% with K-LACGAR and 0.2% with K-LOLAC. Why is there such a difference in values and which value should I use
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When using the Lactose / Galactose Assay Kit (Rapid) (K-LACGAR) for quantitative analysis what level of accuracy and repeatability can be expected?
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What is the detection limit of the Lactose / Galactose Assay Kit (Rapid) (K-LACGAR)?
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What is the linearity of the Lactose / Galactose Assay Kit (Rapid) (K-LACGAR)?
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Is the Lactose/Sucrose/D-Glucose Assay Kit (K-LACSU) suitable for analysis of lactose in dairy by-products?
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Is the Lactose/Sucrose/D-Glucose Assay Kit (K-LACSU) suitable for analysis of sucrose in wort and during fermentation?
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With the Lactose/Sucrose/D-Glucose Assay Kit (K-LACSU), is it possible to measure lactose alone in a mixture of material in which sucrose and glucose could be present?
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When using the Lactulose Assay Kit (K-LACTUL) for quantitative analysis what level of accuracy and repeatability can be expected?
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What is the detection limit of the Lactulose Assay Kit (K-LACTUL)?
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In the Lactulose Assay Kit (K-LACTUL) some samples generate values of A2 – A1 greater than 0.3. Is this ok?
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What is the difference between the different L-malic acid kits (K-LMAL, K-LMALAF, K-LMALMQ and K-LMALQR)?
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Which L-Malic Acid Kit is recommended for a 96-well microplate format?
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When using the L-malic acid kits (K-LMAL, K-LMALAF, K-LMALMQ and K-LMALQR) for quantitative analysis what level of accuracy and repeatability can be expected?
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When using the L-Malic Acid Assay Kit (Manual Format) (K-LMAL) for quantitative analysis what level of accuracy and repeatability can be expected?
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What is the range of the L-Malic Acid Assay Kit (Manual Format) (K-LMAL)?
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What is the detection limit of the L-Malic Acid Assay Kit (Manual Format) (K-LMAL)?
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When using the L-Malic Acid Assay Kit (MegaQuant™ Format) (K-LMALMQ) for quantitative analysis what level of accuracy and repeatability can be expected?
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What analyser would you recommend for use with the L-Malic Acid Assay Kit (MegaQuant™ Format) (K-LMALMQ)?
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What is the range of the L-Malic Acid Assay Kit (MegaQuant™ Format) (K-LMALMQ)?
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When using the Maltose/Sucrose/D-Glucose Assay Kit (K-MASUG) for quantitative analysis what level of accuracy and repeatability can be expected?
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What is the detection limit of the Maltose/Sucrose/D-Glucose Assay Kit (K-MASUG)?
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Can the Maltose/Sucrose/D-Glucose Assay Kit (K-MASUG) specifically measure maltose in the presence of maltitol and maltotriose?
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When using the D-Mannitol Assay Kit (K-MANOL) for quantitative analysis what level of accuracy and repeatability can be expected?
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What is the detection limit of the D-Mannitol Assay Kit (K-MANOL)?
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What is the linearity of the D-Mannitol Assay Kit (K-MANOL)?
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When using the D-Mannose/D-Fructose/D-Glucose Assay kit (K-MANGL) for quantitative analysis what level of accuracy and repeatability can be expected?
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What is the detection limit of the D-Mannose/D-Fructose/D-Glucose Assay kit (K-MANGL)?
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What is the linearity of the D-Mannose/D-Fructose/D-Glucose Assay kit (K-MANGL)?
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When using the Phytic Acid Assay Kit (K-PHYT) for quantitative analysis what level of accuracy and repeatability can be expected?
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What is the detection limit of the Phytic Acid Assay Kit (K-PHYT)?
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What is the linearity of the Phytic Acid Assay Kit (K-PHYT)?
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When using the Primary Amino Nitrogen Assay Kit (K-PANOPA) for quantitative analysis what level of accuracy and repeatability can be expected?
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What kits are recommended to measure yeast assimilable nitrogen (YAN) during wine production?
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What is the detection limit of the Primary Amino Nitrogen Assay Kit (K-PANOPA)?
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Why does the calculation for the K-PDCAAS kit only use the amino acids, Proline, Lysine, Histidine and Arginine?
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Why is the molecular weight of water subtracted for the calculation of amino acids for the K-PDCAAS assay kit?
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How is it possible to obtain values above 1 for in vitro digestibility and amino acid score using the K-PDCAAS calculation?
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When using the Raffinose/D-Galactose Assay Kit (K-RAFGA) for quantitative analysis what level of accuracy and repeatability can be expected?
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What is the detection limit of the Raffinose/D-Galactose Assay Kit (K-RAFGA)?
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What is the linearity of the Raffinose/D-Galactose Assay Kit (K-RAFGA)?
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When using the L-Rhamnose Assay Kit (K-RHAMNOSE) for quantitative analysis what level of accuracy and repeatability can be expected?
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What is the detection limit of the L-Rhamnose Assay Kit (K-RHAMNOSE)?
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What is the linearity of the L-Rhamnose Assay Kit (K-RHAMNOSE)?
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When using the D-Sorbitol/Xylitol Assay Kit (K-SORB) for quantitative analysis what level of accuracy and repeatability can be expected?
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What is the detection limit of the D-Sorbitol/Xylitol Assay Kit (K-SORB)?
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What is the linearity of the D-Sorbitol/Xylitol Assay Kit (K-SORB)?
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What might cause below specification Starch Damage values for the control flour when using the Starch Damage Assay Kit (K-SDAM)?
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Can the Starch Damage Assay Kit (K-SDAM) be used to analyse pasta samples?
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Is there any particular step or enzyme in the Starch Damage Assay Kit (K-SDAM) which may cause reproducibility problems?
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Is it possible to check if the resistant starch values for my samples are correct when analysed using the Resistant Starch Assay Kit (K-RSTAR)?
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What type of the resistant starch is provided as a control with the Resistant Starch Assay Kit (K-RSTAR)?
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What is the reproducibility of the Resistant Starch Assay Kit (K-RSTAR)?
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Does freeze drying affect the resistant starch content for analysis using K-RAPARS?
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In the Resistant Starch (rapid) (K-RAPRS) Assay Protocol, how should samples be stored after "Step E (a) x"?
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Is it possible to use lower centrifuge speeds than those stated in the Resistant Starch (rapid) (K-RAPRS) Assay Protocol?
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Why does the quadruplicate glucose control in your Total Starch Assay Kit (AA/AMG) (K-TSTA) have to be incubated?
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Why are duplicate sample measurements recommend when using the Total Starch Assay Kit (AA/AMG) (K-TSTA)?
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Does the Regular Maize Starch that is supplied with the Total Starch Assay Kit (AA/AMG) (K-TSTA) need to be analysed by pre-treating with DMSO? How should the Regular Maize Starch Control be stored?
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When using the Succinic Acid Assay Kit (K-SUCC) for quantitative analysis what level of accuracy and repeatability can be expected?
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What is the detection limit of the Succinic Acid Assay Kit (K-SUCC)?
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What is the linearity of the Succinic Acid Assay Kit (K-SUCC)?
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I am comparing the results from Sucrose/Fructose/D-Glucose Assay Kit (K-SUFRG) with results obtained by HPLC, and getting different values. Which method works best in general for food and beverage samples?
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When using the Sucrose/Fructose/D-Glucose Assay Kit (K-SURFG) for quantitative analysis what level of accuracy and repeatability can be expected?
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When using the Sucrose/Fructose/D-Glucose Assay Kit (K-SUFRG) for quantitative analysis what level of accuracy and repeatability can be expected?
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What concentration of acetaldehyde in the sample causes interference in the Total Sulfite Assay Kit (K-TSULPH & K-SULPH)?
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How stable are the sulfite standards and samples used in the Total and Free Sulfite Assay Kit (K-TSULPH & K-SULPH)?
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When using the Total and Free Sulfite Assay Kit (K-TSULPH & K-SULPH) for quantitative analysis what level of accuracy and repeatability can be expected?
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Why are results for sulfite standards ~ 20% lower than expected when using the Total Sulfite Assay Kit (Enzymatic) (K-ETSULPH)?
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Is an absorbance change observed of 0.1-0.2 for the blank sample normal when using the Total Sulfite Assay Kit (Enzymatic) (K-ETSULPH)?
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When using the Total Sulfite Assay Kit (Enzymatic) (K-ETSULPH) for quantitative analysis what level of accuracy and repeatability can be expected?
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What concentration of acetaldehyde in the sample causes interference in the total sulfite (TSO2) assay of the Total and Free Sulfite Assay Kit (K-SULPH)?
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How stable are the sulfite standards and samples used with the Total and Free Sulfite Assay Kit (K-SULPH) ?
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When using the Total and Free Sulfite Assay Kit (K-SULPH) for quantitative analysis what level of accuracy and repeatability can be expected?
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When using the Tartaric Acid Assay Kit (K-TART) for quantitative analysis what level of accuracy and repeatability can be expected?
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What is the detection limit of the Tartaric Acid Assay Kit (K-TART)?
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What is the linearity of the Tartaric Acid Assay Kit (K-TART)?
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When using the Urea/Ammonia Assay Kit (Rapid) (K-URAMR) for quantitative analysis what level of accuracy and repeatability can be expected?
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What is the detection limit of the Urea/Ammonia Assay Kit (Rapid) (K-URAMR)?
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What is the linearity of the Urea/Ammonia Assay Kit (Rapid) (K-URAMR)?
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How does the value for endo-xylanase activity obtained with the XylX6 assay compare to the value obtained using reducing sugar assays with polysaccharide substrates?
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How to determine the relevant dilution buffer / extraction buffer in K-XylX6
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Why is 0.5 mg/mL BSA added to the extraction buffer in K-XylX6?
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How are enzymes that are supplied in ammonium sulphate (sulfate) used?
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What is the substrate specificity of each of the Megazyme α-L-arabinofuranosidases?
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How should ammonium sulphate enzyme suspensions be made soluble?
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Why does the Amyloglucosidase Assay Reagent (R-AMGR3) contain PNP-beta-D-maltoside and not PNP-alpha-D-maltoside, since amyloglucosidase is specific for alpha-1,4-D-linked glucans?
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What is the definition of units for Amyloglucosidase (E-AMGDF)?
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In what absorbance range should I use Amyloglucosidase (E-AMGDF) with Amyloglucosidase Assay Reagent (R-AMGR3)?
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Can beta-amylase (E-BARBL/E-BARBP) be used at pH 8 (in order to minimise the alpha-amylase activity). Do you have a buffer to suggest?
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Is the beta-amylase (E-BARBL/E-BARBP) free from other activities?
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What is the relative activity of the liquid (E-BARBL) and powder (E-BARBP) forms of your barley beta-amylase?
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What is the identity of the cellulase (E-CELTR), is it EGI or EGII?
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Does the cellulase (E-CELTR) have any contaminating activities, especially xylanase activity?
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Are there any highly purified enzyme preparations for hydrolysis of cellulose and callose on live plants?
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Why is calcium added to the assay buffer that is used with the Amalyzyme tablets (T-AMZ)?
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Are Cibacron-blue amylose tablets the same as Amylazyme tablets (T-AMZ)?
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How do I measure alpha-amylase in malted wheat? Should I use Format 1 or 2 from the Amylazyme (T-AMZ) booklet?
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Can I use a standard curve generated for an earlier batch of Cellazyme C tablets (T-CCZ) with a new batch of tablets?
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Should the Cellazyme C tablets (T-CCZ) be mottled in colour? The moisture content also seems to be higher than a previous batch.
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Can we run the Cellazyme C tablet method (T-CCZ) at pH 6.2 instead of pH 4.5?
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Can I use a standard curve generated for an earlier batch of Cellazyme T tablets (T-CTZ) with a new batch of tablets?
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Should the Cellazyme T tablets (T-CTZ) be mottled in colour? The moisture content also seems to be higher than a previous batch.
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Can we run the Cellazyme T tablet method (T-CTZ) at pH 6.2 instead of pH 4.5?
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What is this limit-dextrinase as measured by Limit-Dextrizyme tablet (T-LDZ)? Is it pullulanase or isoamylase or alpha-glucosidase or a combination of all the above?
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Do you have an assay that permits the assay of pullulanase in the presence of glucoamylase?
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In the Limit-Dextrizyme Tablet assay the absorbance value is higher than 1.6. The standard curve in the product datasheet only reaches 1.6. therefore I cannot convert this to milli-units/assay.
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What product is recommended to measure activity of proteases such as trypsin or chymotrypsin?
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What is the amount of dye (in milligrams) in the Protazyme Tablets (T-PRAK)?
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Is it a requirement to know what type of protease is being measured in order to use Protazyme tablet method (T-PRAK)? Must it be assumed that only fungal proteases are present in bread improvers?
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What product is recommended to measure activity of proteases such as trypsin or chymotrypsin?
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What is the amount of dye (in milligrams) in the Protazyme Tablets (T-PROL)?
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Is it a requirement to know what type of protease is being measured in order to use Protazyme tablet method (T-PROL)? Must it be assumed that only fungal proteases are present in bread improvers?
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Is it possible to assay brewer’s malt for xylanase using Xylanase AX tablets (T-XAX)?
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Is a method available to check for arabinoxylanase in concentrated Aspergillus and Trichoderma cellulase to be used in baking improvers?
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Why does a higher buffer strength (> 100 mM) inhibit the hydrolysis of AZCL-Xylan in the Xylazyme AX tablet method (T-XAX)?
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Why is Azo-Carob galactomannan (S-ACGLM) is prepared at 2% w/v for the assay, but carob galactomannan is used at only 0.2% w/v for the generation of the standard curve? Which concentration should I use?
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Is 590 nm the best wavelength to read at if I am using Azo-Carob galactomannan (S-ACGLM)?
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In your data sheet the Azo-Carob Galactomannan (S-ACGLM) is reported to be partially depolymerised, is this achieved by enzymatic or acid hydrolysis?
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Are Azo-Barley Glucan (S-ABG100) & Azo-Xylan the best substrates to analyse Trichoderma reseei xylanase and beta-glucanase in animal feed?
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Do you have a typical figure for the ratio of (1-3) to (1-4) linkages in the beta-glucan you use in the Azo-Barley beta-Glucan?
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What is the concentration of Azo-Barley glucan as supplied in liquid form? Also, can this substrate be used to make an agar plate pH 7.0–7.2?
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What kind of dye is used to produce Red Pullulan (S-RPUL); is it the same dye that is used in the arabinan substrate?
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Can Red Pullulan (S-RPUL) substrate be used to assay isoamylase?
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Why is 0.5 M KCI added to the substrate, Red Pullulan (S-RPUL)?
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What chromogenic substrate do you recommend to use in petri dish plates for detection of xylanase activity, and what is the principle of the method?
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Results obtained with Azo-Xylan (Birchwood) (S-AXBL / S-AXBP) seem unstable, and have dropped from 10,000 units to 850 units after 3 weeks?
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I require 4-0-Methyl-D-Glucurono-D-Xylan-Remazol Brilliant Blue R with a dye content of ~13%. Is Azo-Xylan (Birchwood) (S-AXBL / S-AXBP) suitable?
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Can you provide detailed information about the rye flour arabinoxylan (P-RAXY)?
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I found only 64% pentosan when I performed sulphuric acid hydrolysis on the product rye flour arabinoxylan (P-RAXY). Some sugars may have been destroyed in the hydrolysis. Have you anlaysed the sugar composition of your product?
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What are the average molecular weights of the high, medium and low viscosity arabinoxylans P-WAXYL, P-WAXYM & P-WAXYH?
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Does your Wheat Arabinoxylan have any ferulic acid substitutions?
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Are your arabinoxylans and rhamnogalacturonans O-acetylated?
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Will boiling oat beta-glucan (P-BGOM) under neutral conditions cause hydrolysis of this polysaccharide?
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Which method have you used for measuring molecular weight of barley beta-glucan?
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Which method was used to determine the Molecular weights of the β-glucan standards (P-MWBGS)?
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What is the degree of polymerisation of Mannan (P-MANIV) and does it contain any small soluble oligosaccharides?
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How have you extracted the mannan (P-MANIV) from Ivory Nut?
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If I want to degrade mannan by Nelson / Somogyi method in order to determine the amount of mannose, which product is appropriate to this assay and is there a better method than hydrolysis?
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Acetaldehyde (K-ACHYD) Certificate of Analysis
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Acetic Acid (ACS Analyser Format) (K-ACETAF) Certificate of Analysis
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Acetic Acid (ACS Manual Format) (K-ACET) Certificate of Analysis
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Acetyl-CoA synthetase (Bacillus subtilis) (E-ACSBS) Certificate of Analysis
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endo-α-N-Acetylgalactosaminidase (Enterococcus faecalis) (E-OGLYEF) Certificate of Analysis
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α-N-Acetylgalactosaminidase (microbial) (E-ANAGM) Certificate of Analysis
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Blocked 4-nitrophenyl-α-maltoheptaoside (O-BPNPC7) Certificate of Analysis
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β-Naphthyl-β-maltotrioside (O-NAPC3-100) Certificate of Analysis
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4-Nitrophenyl-β-maltotrioside (O-PNPC3-100) Certificate of Analysis
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Amylazyme Tablets (T-AMZ) Certificate of Analysis
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Amylazyme BG Tablets (T-AMZBG-200T) Certificate of Analysis
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Amylazyme Red Tablets (T-AMZRD) Certificate of Analysis
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AZCL-Amylose (I-AZAMY) Certificate of Analysis
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AZCL-Amylose (fine) (I-AZAMYF) Certificate of Analysis
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RedCL-Amylose (fine) (I-RCLAMYF) Certificate of Analysis
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Red Debranched Arabinan (Sugar Beet) (S-RDAR) Certificate of Analysis
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Red Starch (S-RSTAR) Certificate of Analysis
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Azo-CM-Cellulose (Liquid) (S-ACMCL) Certificate of Analysis
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Panose (O-PAN) Certificate of Analysis
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Isopanose (O-IPAN) Certificate of Analysis
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63-α-D-Glucosyl-maltotriose (O-GMT) Certificate of Analysis
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Amylose (potato) (P-AMYL) Certificate of Analysis
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Arabinan (Sugar Beet) (P-ARAB) Certificate of Analysis
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Debranched Arabinan (Sugar Beet) (P-DBAR) Certificate of Analysis
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Acetaldehyde (K-ACHYD) SDS
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Acetic Acid (ACS Manual Format) (K-ACET) SDS
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Acetic Acid (ACS Analyser Format) (K-ACETAF) SDS
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Acetyl-CoA synthetase (Bacillus subtilis) (E-ACSBS) SDS
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endo-α-N-Acetylgalactosaminidase (Enterococcus faecalis) (E-OGLYEF) SDS
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α-N-Acetylgalactosaminidase (microbial) (E-ANAGM) SDS
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Blocked 4-nitrophenyl-α-maltoheptaoside (O-BPNPC7) SDS
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β-Naphthyl-β-maltotrioside (O-NAPC3-100) SDS
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4-Nitrophenyl-β-maltotrioside (O-PNPC3-100) SDS
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Amylazyme Tablets (T-AMZ) SDS
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Amylazyme BG Tablets (T-AMZBG-200T) SDS
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Amylazyme Red Tablets (T-AMZRD) SDS
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Red Debranched Arabinan (Sugar Beet) (S-RDAR) SDS
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Red Starch (S-RSTAR) SDS
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Azo-CM-Cellulose (Liquid) (S-ACMCL) SDS
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Amylose (potato) (P-AMYL) SDS
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Arabinan (Sugar Beet) (P-ARAB) SDS
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Debranched Arabinan (Sugar Beet) (P-DBAR) SDS
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Acetic Acid Standard Solution (1.8 g/L) (AS-ACET) SDS
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Ammonia Standard Solution (0.1 g/L) (AS-AMIA) SDS
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Citric Acid Standard Solution (3.0 g/L) (AS-CITR) SDS