The limit of detection (LoD) of the S-AWAXL assay method was calculated as 7 mU/assay (14 mU/mL) for A. niger xylanase
This detection limit is dictated by the spectrophotometric response and corresponds to an absorbance difference (ΔA) of 0.02 between reaction and blank absorbance values.
The limit of quantification (LoQ) of the S-AWAXL assay method was calculated as 20 mU/assay (40 mU/mL) for A. niger xylanase
This quantification limit is dictated by the spectrophotometric response and corresponds to an absorbance difference (ΔA) of 0.1 between reaction and blank absorbance values.