Ascorbic Acid Assay Kit (L-Ascorbate) (K-ASCO)
Yes. The Ascorbic Acid Assay Kit (L-Ascorbate)
assay kit has been
optimised for general autoanalyser use, however it is important to ensure that
the req...
Wed, 30 Mar, 2016 at 3:57 PM
When the L-ascorbic acid
standard is made up as described in the Ascorbic Acid Assay Kit (L-Ascorbate)
data booklet (0.15 g/L and 0.1
mL used in the ass...
Wed, 30 Mar, 2016 at 3:58 PM
Yes.
Dehydroascorbic acid can be converted to ascorbic acid by addition of DTT
to the sample at final concentration of 1 mM followed by incubation at roo...
Wed, 30 Mar, 2016 at 4:01 PM
Yes. Ascorbic acid
is not very stable and can lose as much as ~ 25% within 3 hours when stored at
room temperature in light. The ascorbic acid should be...
Wed, 30 Mar, 2016 at 4:02 PM
Yes. For solid fruits the following procedure is recommended: Accurately weigh approximately 4 g of sample and homogenise in 10 mL of 1 M potassium phosphat...
Thu, 10 May, 2018 at 3:20 PM
Stability studies on ascorbic acid extraction at Megazyme showed that metaphosphoric acid plus EDTA was very effective at stabilising ascorbic acid. The pre...
Thu, 10 May, 2018 at 3:20 PM
The test kit is extremely
accurate – at Megazyme the quality control criteria for accuracy and
repeatability is to be within 2% of the expected value usin...
Wed, 30 Mar, 2016 at 3:52 PM
The assay is linear over the range of 0.5 to 30 μg of ascorbic acid per assay. The standard of 1.5mg/mL (when prepared as per kit booklet) is equivalent to ...
Thu, 10 May, 2018 at 3:20 PM
The detection limit is 0.175 mg/L, which is derived from an absorbance difference of 0.010 and the maximum sample volume of 1.50 mL. Product Page (K-ASCO)
Thu, 10 May, 2018 at 3:20 PM
