Ascorbic Acid Assay Kit (L-Ascorbate) (K-ASCO)

Is it possible to adapt the Ascorbic Acid Assay Kit (L-Ascorbate) (K-ASCO) for use on various auto-analysers?
Yes. The Ascorbic Acid Assay Kit (L-Ascorbate) assay kit has been optimised for general autoanalyser use, however it is important to ensure that the req...
Wed, 30 Mar, 2016 at 3:57 PM
What absorbance value should be attained using the ascorbic acid standard supplied with Ascorbic Acid Assay Kit (L-Ascorbate) (K-ASCO)?
When the L-ascorbic acid standard is made up as described in the Ascorbic Acid Assay Kit (L-Ascorbate) data booklet (0.15 g/L and 0.1 mL used in the ass...
Wed, 30 Mar, 2016 at 3:58 PM
Is it possible to measure dehydroascorbic acid with the Ascorbic Acid Assay Kit (L-Ascorbate) (K-ASCO)?
Yes. Dehydroascorbic acid can be converted to ascorbic acid by addition of DTT to the sample at final concentration of 1 mM followed by incubation at roo...
Wed, 30 Mar, 2016 at 4:01 PM
The results for ascorbic acid standard in the Ascorbic Acid Assay Kit (L-Ascorbate) (K-ASCO) seem to fluctuate by as much as 15%. Is there an explanation for this?
Yes. Ascorbic acid is not very stable and can lose as much as ~ 25% within 3 hours when stored at room temperature in light. The ascorbic acid should be...
Wed, 30 Mar, 2016 at 4:02 PM
Is it possible to use Ascorbic Acid Assay Kit (L-Ascorbate) (K-ASCO) to measure ascorbic acid in fruits and what extraction procedure is recommended?
Yes. For solid fruits the following procedure is recommended: Accurately weigh approximately 4 g of sample and homogenise in 10 mL of 1 M potassium phosphat...
Thu, 10 May, 2018 at 3:20 PM
What buffer can I use to extract and stabilise ascorbic acid when using the Ascorbic Acid Assay Kit (L-Ascorbate) (K-ASCO)?
Stability studies on ascorbic acid extraction at Megazyme showed that metaphosphoric acid plus EDTA was very effective at stabilising ascorbic acid. The pre...
Thu, 10 May, 2018 at 3:20 PM
When using the Ascorbic Acid Assay Kit (L-Ascorbate) (K-ASCO) for quantitative analysis what level of accuracy and repeatability can be expected?
The test kit is extremely accurate – at Megazyme the quality control criteria for accuracy and repeatability is to be within 2% of the expected value usin...
Wed, 30 Mar, 2016 at 3:52 PM
What is the range of the Ascorbic Acid Assay Kit (L-Ascorbate) (K-ASCO)?
The assay is linear over the range of 0.5 to 30 μg of ascorbic acid per assay. The standard of 1.5mg/mL (when prepared as per kit booklet) is equivalent to ...
Thu, 10 May, 2018 at 3:20 PM
What is the limit of detection of the Ascorbic Acid Assay Kit (L-Ascorbate) (K-ASCO)?
The detection limit is 0.175 mg/L, which is derived from an absorbance difference of 0.010 and the maximum sample volume of 1.50 mL. Product Page (K-ASCO)
Thu, 10 May, 2018 at 3:20 PM