Megazyme Knowledge Base

Yes. The Ascorbic Acid Assay Kit (L-Ascorbate) assay kit has been optimised for general autoanalyser use, however it is important to ensure that the required settings are inputted and that the instrument is able to perform the required number of additions for the automated assay e.g. sample, R1, R2, R3 if applicable. If you require further information regarding the set-up of the Megazyme test kits in autoanalyser format for use with a particular auto-analyser instrument please fill out the Autoanalyser Parameters form and send this to Megazyme via "Ask A Scientist" listing the test kits which you wish to perform along with details of the type of samples you wish to test.

Suggested auto-analyser format for K-ASCO:

 Prepare the reagents as described in the K-ASCO data booklet then prepare R1 and R2 as follows:
Preparation of R1: (90 assays)

Preparation of R2: (90 assays)

EXAMPLE METHOD:
R1: 0.260 mL
Sample: ~ 0.005 mL
R2: 0.05 mL

 

Note: For accurate measurement of ascorbic acid each sample requires two measurements, one with AAO (bottle 4) present and one with AAO (bottle 4) absent.
In the auto-analyser format described above there is no option to read A1 as in the cuvette assay. In this instance use an ascorbic acid calibration curve to calculate results. Alternatively add 0.025 mL of bottle 2 and 3 separately to mimic the cuvette assay and allow the reading A1 (this will require 3 reagent additions) OR it may be possible to add the MTT (bottle 2) with R1 as follows:

 

Preparation of R1: (90 assays) 

Preparation of R2: (90 assays)

EXAMPLE METHOD:
R1: 0.285 mL
Sample: ~ 0.005 mL
R2: 0.025 mL 

Megazyme routinely uses this kit on Chemwell-T and Chemwell 2910 auto-analysers, which are also available for sale through Megazyme.

Product Page (K-ASCO)