Is it possible to measure dehydroascorbic acid with the Ascorbic Acid Assay Kit (L-Ascorbate) (K-ASCO)?

Modified on Fri, 24 Sep, 2021 at 12:24 PM

Yes. Dehydroascorbic acid can be converted to ascorbic acid by addition of DTT to the sample at final concentration of 1 mM followed by incubation at room temperature for 10 minutes. The difference in the ascorbic acid content of the DTT treated sample compared to the untreated sample is proportional to the dehydroascorbic acid content of the sample. 


Product Page (K-ASCO)

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