Lactose/Galactose Assay Kit (Rapid) (K-LACGAR)

When using the Lactose / Galactose Assay Kit (Rapid) (K-LACGAR) for quantitative analysis what level of accuracy and repeatability can be expected?
The Lactose/Galactose Assay Kit (Rapid) is extremely accurate – at Megazyme the quality control criteria for accuracy and repeatability is to be within 2...
Mon, 4 Apr, 2016 at 3:56 PM
What is the detection limit of the Lactose / Galactose Assay Kit (Rapid) (K-LACGAR)?
For Procedure A the detection limit of the Lactose/Galactose Assay Kit (Rapid)  is 2.96 mg/L of lactose and 1.48 mg/L of D-galactose, which is derived fr...
Fri, 3 Jun, 2016 at 10:25 AM
What is the linearity of the Lactose / Galactose Assay Kit (Rapid) (K-LACGAR)?
The Lactose/Galactose Assay Kit (Rapid)  is linear over the range 8 to 160 µg of lactose or 4 to 80 µg of D-galactose per assay.  Using a 0.10 mL assay vol...
Mon, 4 Apr, 2016 at 3:58 PM
In the Lactose / Galactose Assay Kit (Rapid) (K-LACGAR) why is the borohydride reduction step required in procedure B?
Procedure B is required for “low-lactose” or “lactose-free” samples containing high levels of monosaccharides.  Generally, these types of samples con...
Mon, 4 Apr, 2016 at 3:59 PM
Can the Lactose / Galactose Assay Kit (Rapid) (K-LACGAR) be used for the reliable detection of lactose in bakery products at a level of approximately 100 mg per 100g?
Yes, this is possible. Here are two options for sample preparation methods for pastry products: 1. Mill or homogenise sample materials. Weigh out a repr...
Mon, 4 Apr, 2016 at 4:00 PM
For the Lactose/Galactose Assay Kit (Rapid) why is the detectable range of lactose is 0.008 - 0.16 g/L using a 1 mL sample but the detection limit is given as 0.00296 g/L for lactose?
The linear range of 0.008 - 0.16 g/L is based on the recommended minimum absorbance change of 0.1, however some users are comfortable working below this l...
Mon, 4 Apr, 2016 at 4:02 PM
How do I know which procedure to use for my sample(s) in the Lactose/Galactose Assay Kit (Rapid) (K-LACGAR)?
As a general rule: Procedure A is used for samples that are known to contain low levels of free galactose. Procedure B is used for samples that are known ...
Mon, 4 Apr, 2016 at 4:03 PM
Can the Lactose/Galactose Assay Kit (Rapid) (K-LACGAR) be used to test solid samples using PROCEDURE B: (For “low-lactose” or “lactose-free” samples containing high levels of monosaccharides)?
Step 1: Add 1 g of sample (or homogenised sample) to 4 mL of water, mix then add 1 mL of 10 mg/mL sodium borohydride (dissolved in 50 mM NaOH and less tha...
Mon, 4 Apr, 2016 at 4:04 PM