The linear range of 0.008 - 0.16 g/L is based on the recommended minimum absorbance change of 0.1, however some users are comfortable working below this level hence the limit of detection is based on a 1 mL sample volume and minimum absorbance change of 0.02. The sample volume can be altered; for samples containing low concentrations of lactose the sample volume can be increased to up to 1 mL however the distilled water volume must be altered accordingly so that the final assay volume is not altered, otherwise the calculation will be affected (see page 7 of the K-LACGAR booklet). For concentrated samples these should be diluted in distilled water and the dilution factor included into the calculation.
For the Lactose/Galactose Assay Kit (Rapid) why is the detectable range of lactose is 0.008 - 0.16 g/L using a 1 mL sample but the detection limit is given as 0.00296 g/L for lactose?
Modified on: Mon, 4 Apr, 2016 at 4:02 PM
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