Procedure B is required for “low-lactose” or “lactose-free”
samples containing high levels of monosaccharides. Generally, these types of samples contain high
levels of “free” galactose which causes a high background and reduces the
dynamic range available to measure the galactose that is released from lactose
in the test. To avoid this the borohydride step is used to reduce the free
galactose.
In the Lactose / Galactose Assay Kit (Rapid) (K-LACGAR) why is the borohydride reduction step required in procedure B?
Modified on: Mon, 4 Apr, 2016 at 3:59 PM
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