Amylose/Amylopectin Assay Kit (K-AMYL) : Megazyme

Amylose/Amylopectin Assay Kit (K-AMYL)

With your Amylose/Amylopectin Assay Kit (K-AMYL) can you calculate the amylose content from the Con A supernatant absorbance without performing the total starch section if you have recorded the weight of the sample?

Amylose is always reported as a percentage of the starch, not as a percentage of the sample weight. The method should be followed as described. Pr...

Wed, 30 Mar, 2016 at 10:49 AM

Can Amylose/Amylopectin Assay Kit (K-AMYL) also be used for total starch determination?

We do not recommend K-AMYL for starch measurement. The aim with this method is to get a representative sample of the starch to get the amylose content. F...

Wed, 30 Mar, 2016 at 10:48 AM

Do you provide any information in your Amylose/Amylopectin Kit (K-AMYL) for the separation of the two starch fractions?

We think that the only effective method to separate amylose and amylopectin is by gel filtration. Product Page (K-AMYL)

Wed, 30 Mar, 2016 at 10:49 AM

When using the Amylose/Amylopectin Kit (K-AMYL) what are the recommendations regarding sample grinding? We have used a Retsch laboratory mill with a 0.5 mm screen. The particle size may be important for starch extraction.

Milling with Retsch 0.5 mm screen is sufficient. Product Page (K-AMYL)

Tue, 22 Oct, 2019 at 4:39 PM

Is the Amylose/Amylopectin Assay Kit (K-AMYL) suitable for measuring potato starch?

The method is suitable for measuring amylose/amylopectin in potato starch. Product Page (K-AMYL)

Wed, 30 Mar, 2016 at 10:52 AM

What is the level of repeatability of the Amylose/Amylopectin Kit (K-AMYL)?

Reproducibility of this kit is +/- 2 to 4% of the expected value. However this can vary depending on analyst, equipment and sample. Product Page (K-...

Wed, 30 Mar, 2016 at 10:53 AM

How sensitive is your Amylose/Amylopectin Kit (K-AMYL) – can it differentiate between 50/50 and a 55/45 ratio?

Yes, there should be no problem in distinguishing samples with these differences in amylose content. Product Page (K-AMYL)

Wed, 30 Mar, 2016 at 10:54 AM

When using the Amylose/Amylopectin Assay Kit (K-AMYL) the solution (supernatant) is not always clear after centrifuging and after mixing with Con A. Is this OK?

In both cases this is OK and is normal. Product Page (K-AMYL)

Wed, 30 Mar, 2016 at 10:56 AM

What kind of flour is the reference starch sample in the Amylose/Amylopectin Assay Kit (K-AMYL)?

The starch control is high amylose maize starch. Product Page (K-AMYL)

Wed, 30 Mar, 2016 at 10:57 AM

What is the difference between your Amylose/Amylopectin Assay Kit (K-AMYL) and the Total Starch Kit (K-TSTA)? I need to separate amylose from amylopectin in maize plants and quantify each fraction - which kit would be most suitable.

The Amylose/Amylopectin Asay Kit (K-AMYL) is most suitable and employs Con A to selectively precipitate the amylopectin. Product Page (K-AMYL)