Amylose/Amylopectin Assay Kit (K-AMYL) : Megazyme

Amylose/Amylopectin Assay Kit (K-AMYL)

With your Amylose/Amylopectin Assay Kit (K-AMYL) can you calculate the amylose content from the Con A supernatant absorbance without performing the total starch section if you have recorded the weight of the sample?

Wed, 10 Nov, 2021 at 3:07 PM

Can Amylose/Amylopectin Assay Kit (K-AMYL) also be used for total starch determination?

Wed, 10 Nov, 2021 at 3:08 PM

Do you provide any information in your Amylose/Amylopectin Kit (K-AMYL) for the separation of the two starch fractions?

Wed, 10 Nov, 2021 at 3:08 PM

When using the Amylose/Amylopectin Kit (K-AMYL) what are the recommendations regarding sample grinding? We have used a Retsch laboratory mill with a 0.5 mm screen. The particle size may be important for starch extraction.

Wed, 10 Nov, 2021 at 3:08 PM

Is the Amylose/Amylopectin Assay Kit (K-AMYL) suitable for measuring potato starch?

Wed, 10 Nov, 2021 at 3:08 PM

What is the level of repeatability of the Amylose/Amylopectin Kit (K-AMYL)?

Wed, 10 Nov, 2021 at 3:08 PM

How sensitive is your Amylose/Amylopectin Kit (K-AMYL) – can it differentiate between 50/50 and a 55/45 ratio?

Wed, 10 Nov, 2021 at 3:08 PM

When using the Amylose/Amylopectin Assay Kit (K-AMYL) the solution (supernatant) is not always clear after centrifuging and after mixing with Con A. Is this OK?

Wed, 10 Nov, 2021 at 3:09 PM

What kind of flour is the reference starch sample in the Amylose/Amylopectin Assay Kit (K-AMYL)?

Wed, 10 Nov, 2021 at 3:10 PM

In the Amylose/Amylopectin Assay Kit (K-AMYL) what modifications for steps A2 – A7 can be used for problem samples which do not appear to dissolve fully in DMSO (e.g. rice flours, potato starch)?

Wed, 10 Nov, 2021 at 3:10 PM

What is the difference between your Amylose/Amylopectin Assay Kit (K-AMYL) and the Total Starch Kit (K-TSTA)? I need to separate amylose from amylopectin in maize plants and quantify each fraction - which kit would be most suitable.

Wed, 10 Nov, 2021 at 3:12 PM

In the Amylose/Amylopectin Assay Kit (K-AMYL) procedure is the centrifugation speed or temperature more important after the ConA precipitation step, or are they equally important?

Wed, 10 Nov, 2021 at 3:12 PM

Can the Amylose/Amylopectin Assay Kit (K-AMYL) be used to measure total starch?

Wed, 10 Nov, 2021 at 3:13 PM

Is it possible to analyse samples containing fat with the Amylose/Amylopectin kit (K-AMYL)?

Tue, 31 Aug, 2021 at 3:03 PM

Is it possible to determine the amylose content in chemically modified waxy maize starch using K-AMYL?

Mon, 12 Aug, 2019 at 11:39 AM

There are crystals in my GOPOD reagent buffer. Is this normal and will it affect my assay?

Thu, 6 May, 2021 at 12:35 PM

How do you calculate the amount of amylopectin in your sample when using the Amylose/Amylopectin Assay Kit (K-AMYL)?

Mon, 8 Nov, 2021 at 3:20 PM