Amylose/Amylopectin Assay Kit (K-AMYL)

With your Amylose/Amylopectin Assay Kit (K-AMYL) can you calculate the amylose content from the Con A supernatant absorbance without performing the total starch section if you have recorded the weight of the sample?
Amylose is always reported as a percentage of the starch, not as a percentage of the sample weight. The method should be followed as described. Pr...
Wed, 30 Mar, 2016 at 10:49 AM
Can Amylose/Amylopectin Assay Kit (K-AMYL) also be used for total starch determination?
We do not recommend K-AMYL for starch measurement.  The aim with this method is to get a representative sample of the starch to get the amylose content. F...
Wed, 30 Mar, 2016 at 10:48 AM
Do you provide any information in your Amylose/Amylopectin Kit (K-AMYL) for the separation of the two starch fractions?
We think that the only effective method to separate amylose and amylopectin is by gel filtration. Product Page (K-AMYL)
Wed, 30 Mar, 2016 at 10:49 AM
When using the Amylose/Amylopectin Kit (K-AMYL) what are the recommendations regarding sample grinding? We have used a Retsch laboratory mill with a 0.5 mm screen. The particle size may be important for starch extraction.
Milling with Retsch 0.5 mm screen is sufficient. Product Page (K-AMYL)
Wed, 30 Mar, 2016 at 10:51 AM
Is the Amylose/Amylopectin Assay Kit (K-AMYL) suitable for measuring potato starch?
The method is suitable for measuring amylose/amylopectin in potato starch. Product Page (K-AMYL)
Wed, 30 Mar, 2016 at 10:52 AM
What is the level of repeatability of the Amylose/Amylopectin Kit (K-AMYL)?
Reproducibility of this kit is +/- 2 to 4% of the expected value. However this can vary depending on analyst, equipment and sample. Product Page (K-...
Wed, 30 Mar, 2016 at 10:53 AM
How sensitive is your Amylose/Amylopectin Kit (K-AMYL) – can it differentiate between 50/50 and a 55/45 ratio?
Yes, there should be no problem in distinguishing samples with these differences in amylose content. Product Page (K-AMYL)
Wed, 30 Mar, 2016 at 10:54 AM
When using the Amylose/Amylopectin Assay Kit (K-AMYL) the solution (supernatant) is not always clear after centrifuging and after mixing with Con A. Is this OK?
In both cases this is OK and is normal.  Product Page (K-AMYL)
Wed, 30 Mar, 2016 at 10:56 AM
What kind of flour is the reference starch sample in the Amylose/Amylopectin Assay Kit (K-AMYL)?
The starch control is high amylose maize starch. Product Page (K-AMYL)
Wed, 30 Mar, 2016 at 10:57 AM
In the Amylose/Amylopectin Assay Kit (K-AMYL) what modifications for steps A2 – A7 can be used for problem samples which do not appear to dissolve fully in DMSO (e.g. rice flours, potato starch)?
We suggest that you make the following changes: 1. A2 – use 2 mL DMSO 2. A4 – use total of 12 mL Ethanol (4 + 8– need longer tubes) 3. A6 – use...
Wed, 30 Mar, 2016 at 10:58 AM