We have three soluble substrates for the measurement of xylanase activity. However we recommend using the Azo-Xylan (Birchwood) (S-AXBP or S-AXBL). All of the Azo substrates are blue coloured and the reaction is determined by adding an alcohol solution followed by centrifugation. In a petri dish assay, as the substrate is hydrolysed, it would diffuse more rapidly than the native substrate, giving zones of clearing.
What chromogenic substrate do you recommend to use in petri dish plates for detection of xylanase activity, and what is the principle of the method?
Modified on: Fri, 25 Mar, 2016 at 10:47 AM
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