Amyloglucosidase acts reasonably rapidly on maltose linked to PNP, but not on glucose linked to PNP (alpha-linked). Therefore, we have prepared a reagent containing maltose beta-linked to PNP, and this is used in the presence of excess quantities of beta-glucosidase. When AMG releases the terminal glucose, the beta-glucosidase instantaneously hydrolyses the second glucosidic bond, releasing free PNP which is measured in the assay.
Why does the Amyloglucosidase Assay Reagent (R-AMGR3) contain PNP-beta-D-maltoside and not PNP-alpha-D-maltoside, since amyloglucosidase is specific for alpha-1,4-D-linked glucans?
Modified on: Wed, 30 Mar, 2016 at 12:00 PM
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