Amyloglucosidase Assay Reagent (R-AMGR3)

Why does the Amyloglucosidase Assay Reagent (R-AMGR3) contain PNP-beta-D-maltoside and not PNP-alpha-D-maltoside, since amyloglucosidase is specific for alpha-1,4-D-linked glucans?
Amyloglucosidase acts reasonably rapidly on maltose linked to PNP, but not on glucose linked to PNP (alpha-linked). Therefore, we have prepared a reagent...
Wed, 30 Mar, 2016 at 12:00 PM
Can the Amyloglucosidase Reagent (R-AMGR3) be used to measure Amyloglucosidase (E-AMGDF) levels in malted barley and what is a suitable extraction procedure to use for ground malt?
The amyloglucosidase (AMG) present in ground malt is most probably of fungal origin, i.e. A. niger.  The flour should be extracted with 100 mM sodium ace...
Wed, 30 Mar, 2016 at 12:01 PM
In what absorbance range should I use Amyloglucosidase (E-AMGDF) with Amyloglucosidase Assay Reagent (R-AMGR3)?
The Amyloglucosidase Assay Reagent (R-AMGR3) is optimised for use in absorbance range 0.2-0.9. Dilute enzyme to get within this range. Product Pag...
Wed, 30 Mar, 2016 at 12:01 PM
Can Amyloglucosidase Assay Reagent (R-AMGR3) be used to measure amyloglucosidase (E-AMGDF) in the presence of α-glucosidase?
No, the substrate is acted on by both of these enzymes and significant amounts of α-glucosidase would interfere. However, most amyloglucosidase preparatio...
Wed, 30 Mar, 2016 at 12:01 PM