Which extraction / dilution buffer should I use for the Cellulase Assay Kit (K-CELLG5)?

Modified on Thu, 9 Sep, 2021 at 5:48 PM

The choice of buffer depends on the pH optima of the enzyme being measured and therefore the buffer that is closest in pH to the enzyme pH optima should be used. Usually cellulases of fungal origin with have a lower pH optima e.g. ~ 4 to 4.5 while cellulases of bacterial  origin with have a higher pH optima e.g. ~ 6 to 7.5.

If the origin of the cellulase being measured is unknown then the recommendation is to to test the enzyme with both buffers and see which buffer generates the higher activity.


Product Page (K-CellG5)

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