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Why is Azo-Carob galactomannan (S-ACGLM) is prepared at 2% w/v for the assay, but carob galactomannan is used at only 0.2% w/v for the generation of the standard curve? Which concentration should I use?
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Is 590 nm the best wavelength to read at if I am using Azo-Carob galactomannan (S-ACGLM)?
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In your data sheet the Azo-Carob Galactomannan (S-ACGLM) is reported to be partially depolymerised, is this achieved by enzymatic or acid hydrolysis?