β-Glucan Assay Kit (Mixed Linkage) (K-BGLU)

What is the Mixed Linkage β-Glucan Assay Kit (K-BGLU) used to measure? What is the specificity of this kit?
This kit is a highly specific enzymatic procedure for the measurement of cereal 1,3:1,4-β-D-glucans e.g. from oat, barley and wheat. Product Page (K...
Fri, 22 Apr, 2016 at 9:45 AM
What is mixed-linkage β -glucan that you can measure using β-Glucan Assay Kit (K-BGLU)?
The cereal β-glucans (so-called mixed-linkage β-glucans) are linear polysaccharides in which D-glucosyl residues are linked 1,3-β- and 1,4-β-, and the rat...
Fri, 22 Apr, 2016 at 9:45 AM
Is it possible to specifically measure cereal beta-glucan in a mixed material of mushroom/yeast and cereal/grains using the Mixed Linkage β-Glucan Assay Kit (K-BGLU)?
The structures of mushroom and fungal β-glucans are different from the cereal β-glucans. Fungal and yeast cell walls are composed of ≤ 50% 1,3:1,6-β-gluca...
Fri, 22 Apr, 2016 at 9:46 AM
Is it possible to check if the β-glucan values for my samples are correct when analysed using the Mixed Linkage β-Glucan Assay Kit (K-BGLU)?
We highly recommend analysing the oat and barley controls provided with the kit with each set of determinations. If values received for the controls are a...
Fri, 22 Apr, 2016 at 9:46 AM
What is the reproducibility of the Mixed Linkage β-Glucan Assay Kit (K-BGLU)?
Standard errors of ± 3% are achieved routinely within our laboratory for oat and barley samples. However it is up to each individual customer to decide if...
Fri, 22 Apr, 2016 at 9:46 AM
What β-glucan content can be measured using the Mixed Linkage β-Glucan Assay Kit (K-BGLU)? What is the linear range of the glucose measurement?
The glucose measurement (incubation with GOPOD Reagent) is linear between 4 and 100 micrograms of glucose per assay, which is an equivalent of 0.35 to 8.5...
Fri, 22 Apr, 2016 at 9:47 AM
What is the lower limit of detection of the Mixed Linkage β-Glucan Assay Kit (K-BGLU)?
We recommend that the smallest differentiating absorbance ΔA should be at least 0.04 with GOPOD Reaction. This is the equivalent of approximately 0.35% of...
Fri, 22 Apr, 2016 at 9:47 AM
Is it possible to measure β-glucan in liquid samples (e.g. oat drinks, fruit juices enriched with β-glucan) using the Mixed Linkage β-Glucan Assay Kit (K-BGLU)? What is the limit of detection for this type of sample?
The procedure (C) for liquid samples is described in the data booklet. The limit of detection is 0.01g/100 mL of β-glucan with ΔA of approximately 0.05 an...
Fri, 22 Apr, 2016 at 9:47 AM
Are there any interfering substances that can affect the performance of Mixed Linkage β-Glucan Assay Kit (K-BGLU)? Is it possible to check this?
The colour production with GOPOD Reagent may be affected by high salt concentrations above 0.2M and possibly by ascorbate. To test for possible effects on...
Fri, 22 Apr, 2016 at 9:48 AM
Will a sample containing sucrose interfere with the final measurement of mixed-linkage β-glucans using β-Glucan Assay Kit (K-BGLU)?
Sucrose will not interfere; the enzymes will not act on sucrose. The sample blank accounts for glucose from any source other than β-glucan. Produc...
Fri, 22 Apr, 2016 at 9:48 AM