The procedure (C) for liquid samples is described in the data booklet. The limit of detection is 0.01g/100 mL of β-glucan with ΔA of approximately 0.05 and 0.28 g/100 mL with ΔA = 1.0. If expected β-glucan content in your sample is higher than 0.28 g/100 mL or received absorbance values for the sample are higher than the absorbance values obtained for glucose standard, the sample solution must be diluted sufficiently with 200 mM sodium acetate buffer (pH 4.0) before the β-glucosidase treatment.
Is it possible to measure β-glucan in liquid samples (e.g. oat drinks, fruit juices enriched with β-glucan) using the Mixed Linkage β-Glucan Assay Kit (K-BGLU)? What is the limit of detection for this type of sample?
Modified on: Fri, 22 Apr, 2016 at 9:47 AM
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