It is necessary to incubate glucose standards (recommended in quadruplicate) with each set of determinations. Firstly, the intensity of the colour response of GOPOD Reagent can change over time. The absorbance value of glucose standards is used in the calculations (F-factor) and therefore it is crucial in order to obtain accurate results. Secondly, glucose standards are required to ensure that GOPOD Reagent is working correctly for glucose determination. 

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