Xylazyme AX Tablets (T-XAX-200T/T-XAX-1000T)

Is it possible to assay brewer’s malt for xylanase using Xylanase AX tablets (T-XAX)?
There is very little xylanase in malt. We suggest you use the same buffer as recommended (25 mM acetate, pH 4.7). You will need to increase incubation ...
Thu, 25 Feb, 2016 at 10:27 AM
Is a method available to check for arabinoxylanase in concentrated Aspergillus and Trichoderma cellulase to be used in baking improvers?
Arabinoxylanase is actually "xylanase". This is the enzyme which depolymerises arabinoxylans. Xylazyme AX can be used for both Aspergillus an...
Tue, 22 Mar, 2016 at 12:28 PM
Why does a higher buffer strength (> 100 mM) inhibit the hydrolysis of AZCL-Xylan in the Xylazyme AX tablet method (T-XAX)?
Salt effects sensitivity by effecting the rate and extent of swelling of the dyed and crosslinked substrate particles. If the swelling of the particles is...
Tue, 22 Mar, 2016 at 12:28 PM
What is the unit definition for xylanase in the Xylazyme tablet methods (T-XAX)?
The units are International Units of activity determined using the Somogyi reducing sugar method. One Unit is the amount of enzyme required to release on...
Thu, 25 Feb, 2016 at 10:39 AM
When using the Xylazyme tablet method (T-XAX), within how many minutes after filtration of the reacted solution should the absorbance be measured?
When using the Xylazyme tablet method (T-XAX), filtration should be performed about 5 minutes after stopping the reaction. For Xylanases with alkaline pH...
Tue, 22 Mar, 2016 at 12:31 PM
What is the accuracy of the Xylazyme tablet method (T-XAX)?
The repeatability for this assays is +/- 7%. Product Page
Thu, 25 Feb, 2016 at 10:45 AM
Is it necessary to generate a new standard curve for the Xylazyme tablet method (T-XAX) if measuring xylanase activity produced by a submerged culture of Aspergillus?
Yes it will be necessary to generate a new standard curve but it is expected that it will be very similar to the A. niger curve in the booklet. Pr...
Tue, 22 Mar, 2016 at 12:31 PM
From our analysis we have found that the optimum temperature for the Xylazyme tablet method (T-XAX) is 50°C instead of 40°C. Can you tell us how you determine the temperature optimum?
We do not give a "temperature optimum" for use of the tablets, we just recommend a "suggested" assay format for different enzymes. We ...
Thu, 25 Feb, 2016 at 10:52 AM