General FAQs - Assay Kits

Are the enzymes that are used in the Megazyme test kits stable when shipped at ambient temperature?
All enzymes used in Megazyme kits have been subject to extensive stability studies and not affected by short term ambient shipping and are of high stability...
Fri, 22 Apr, 2016 at 2:31 PM
Should the pH of the sample be adjusted even for samples in acidic media?
The pH of the assay solution after the sample is added should be the same as that of the assay buffer that is supplied with the kit. Low sample volumes (e.g...
Wed, 30 Mar, 2016 at 10:44 AM
I have some doubts about the appearance/quality of a kit component what should be done?
If there are any concerns with any kit components, the first thing to do is to test the standard sample (control sample) that is supplied with the kit and...
Wed, 30 Mar, 2016 at 10:20 AM
Sometimes a negative absorbance change is obtained for the blank samples, is this normal? Should the real value (negative absorbance change) or “0” be used in the calculation of results?
Sometimes the addition of the last assay component can cause a small negative absorbance change in the blank samples due to a dilution effect and in such ...
Thu, 31 Mar, 2016 at 11:33 AM
Some chemicals are not supplied with Megazyme kits. Can alternative, equivalent chemicals to those that are recommended in the procedure, be used?
Yes, alternative equivalent chemicals can be used as long as the chemical being used is to the same specification as the recommended chemical.  ...
Thu, 2 Jun, 2016 at 10:39 AM
What limits do you recommend for pipette inaccuracy and imprecision?
The pipetting inaccuracy and imprecision limits we recommend regardless of the kit assay are 2% and 5%, respectively. This link may be useful; Gilson ...
Thu, 10 May, 2018 at 3:40 PM
There is an issue with the performance of the kit; the results are not as expected. What should I do?
If you suspect that the Megazyme test kit is not performing as expected such that expected results are not obtained please do the following: Ensu...
Fri, 25 Mar, 2016 at 12:42 PM
Can the manual assay format be scaled down to a 96-well microplate format?
The majority of the Megazyme test kits are developed to work in cuvettes using the manual assay format, however the assay can be converted for use in a 96...
Wed, 30 Mar, 2016 at 10:21 AM
How are results calculated for solid samples?
Solid samples are calculated as g/100 g of original solid material. For solid samples, the concentration at which they have been prepared (g...
Fri, 20 May, 2016 at 10:30 AM
Do samples require any specific sample preparation prior to testing with the kits?
Sample preparation is sample dependent, some samples may be tested directly in the assay or after appropriate dilution, however, some samples may require fu...
Tue, 5 Apr, 2016 at 10:20 AM