General FAQs - Assay Kits & Reagents

Are the enzymes that are used in the Megazyme products stable when shipped at ambient temperature?
Fri, 15 Feb, 2019 at 11:40 AM
Should the pH of the sample be adjusted even for samples in acidic media?
Wed, 30 Mar, 2016 at 10:44 AM
I have some doubts about the appearance/quality of a kit component what should be done?
Wed, 30 Mar, 2016 at 10:20 AM
Sometimes a negative absorbance change is obtained for the blank samples, is this normal? Should the real value (negative absorbance change) or “0” be used in the calculation of results?
Thu, 31 Mar, 2016 at 11:33 AM
Some chemicals are not supplied with Megazyme kits. Can alternative, equivalent chemicals to those that are recommended in the procedure, be used?
Thu, 2 Jun, 2016 at 10:39 AM
What limits do you recommend for pipette inaccuracy and imprecision?
Thu, 10 May, 2018 at 3:40 PM
Why are temperatures in the video method different to the kit booklet?
Wed, 1 Sep, 2021 at 12:41 PM
There is an issue with the performance of the kit; the results are not as expected. What should I do?
Mon, 20 Apr, 2020 at 10:26 AM
Can the manual assay format be scaled down to a 96-well microplate format?
Wed, 30 Mar, 2016 at 10:21 AM
How are results calculated for solid samples?
Fri, 20 May, 2016 at 10:30 AM
Do samples require any specific sample preparation prior to testing with the kits?
Tue, 5 Apr, 2016 at 10:20 AM
Can the test kit be used to measure biological fluids and what sample preparation method should be used?
Tue, 24 Jan, 2017 at 10:55 AM
Is it possible to add a larger volume then 2 μL of enzyme to the microplate assay?
Mon, 4 Apr, 2016 at 2:11 PM
How can I work out how much sample to extract and what dilution of my sample should be used in the kit assay?
Wed, 30 Mar, 2016 at 10:31 AM
What is the period of stability of the enzymes and buffers supplied in the Megazyme test kits?
Mon, 18 Apr, 2016 at 12:36 PM
Can the sensitivity of the kit assay be increased?
Wed, 30 Mar, 2016 at 10:26 AM
How do I dispose of hazardous chemicals that are supplied in Megazyme products?
Fri, 16 Sep, 2016 at 4:55 PM
How much sample should be used for the clarification / extraction of my sample for quantitative analysis in a kit assay?
Wed, 30 Mar, 2016 at 10:32 AM
When using a Megazyme test kit for quantitative analysis what level of accuracy and repeatability can be expected?
Wed, 30 Mar, 2016 at 10:30 AM
What is the expected analytical variation for the Reference Starch that is supplied with the Starch Damage Assay Kit (K-SDAM)?
Mon, 18 Apr, 2016 at 12:29 PM
Must the minimum absorbance change for a sample always be at least 0.1 when using a Megazyme kit assay?
Wed, 30 Mar, 2016 at 10:27 AM
The expiry date / shelf life for the Megazyme test kit has passed can the kit still be used?
Wed, 7 Jun, 2017 at 4:20 PM
What type of cuvettes are recommended for the absorbance readings when using Megazyme assay kits?
Thu, 10 May, 2018 at 3:43 PM
Must sodium azide be added to buffers when they are prepared? Can buffers be prepared without azide?
Thu, 10 May, 2018 at 3:44 PM
Is it possible to perform the NAD(P) coupled assays at a wavelength other than 340 nm?
Thu, 6 Jul, 2017 at 2:47 PM
How are the MegaCalc applications used? The Mega-Calc appears to be locked?
Mon, 14 Oct, 2019 at 1:08 PM
Can I make less than 1 L of GOPOD Reagent at once?
Thu, 10 May, 2018 at 3:45 PM
There are crystals in my GOPOD reagent buffer. Is this normal and will it affect my assay?
Thu, 6 May, 2021 at 12:27 PM
What is the purpose of adding sodium azide to buffers? Must sodium azide be added to buffers when prepared by the user?
Thu, 28 Apr, 2016 at 12:36 PM
Why is there no expiration date on my certificate of analysis?
Tue, 12 Nov, 2019 at 9:28 AM
The amount of kit components does not match and there are some components left over when others are depleted?
Mon, 20 Nov, 2017 at 11:46 AM
How to unprotect MegaCalc sheets?
Fri, 31 Jan, 2020 at 2:42 PM
How should kit components be stored after they are delivered?
Fri, 1 Apr, 2016 at 4:41 PM
The NAD+ solution has developed a yellow tinge stored it at -20C in aliquots. Can it still be used?
Thu, 28 Apr, 2016 at 3:46 PM
How do I know if my sample is interfering with the assay?
Fri, 7 Oct, 2016 at 4:26 PM
Are kit components available as individual products?
Thu, 10 May, 2018 at 3:47 PM
Sample preparation of milk (liquid).
Tue, 3 Oct, 2017 at 10:25 AM
What does the acronym BSA stand for and what is it used for?
Thu, 22 Aug, 2019 at 8:56 AM
The kit assay is not working, what should I do?
Wed, 29 Jan, 2020 at 11:34 AM
Why is a microplate assay is not working with a Megazyme kit?
Wed, 1 Sep, 2021 at 12:45 PM
Is it possible to know if a kit was affected by storage outside recommended conditions?
Thu, 12 Aug, 2021 at 11:54 AM
The Gopod reagents appear to be from a different kit, are these correct?
Thu, 12 Aug, 2021 at 11:57 AM
Is a kit or product usable / reliable after the expiry date?
Thu, 12 Aug, 2021 at 12:51 PM
Does Megazyme supply a kit that measures amylose and beta-glucan at the same time?
Wed, 28 Jul, 2021 at 2:54 PM