The
volume/weight of sample and total volume of the extract can be modified to suit
the sample. This will ultimately be
dictated by the amount of analyte of interest in the sample and may require
empirical determination. For low levels
of analyte the sample:extract volume ratio can be increased (i.e. increase the
sample and/or decrease the total extraction volume).
Alternatively, for samples with low concentrations of analyte, a larger sample volume can be added to the kit assay. When altering the sample volume adjust the distilled water volume added to the assay accordingly so that the total assay volume is not altered.
How much sample should be used for the clarification / extraction of my sample for quantitative analysis in a kit assay?
Modified on: Thu, 11 Nov, 2021 at 10:52 AM
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