Fructan Assay Kit (K-FRUC)
Yes. Product Page (K-FRUCHK) Product Page (K-FRUC)
Thu, 31 Mar, 2016 at 11:40 AM
When measuring fructan in starch containing samples, i.e. maize, is there a possibility of incomplete starch hydrolysis in step B of the Fructan Assay (K-FRUC) ?
From our experience, the level of beta-amylase/pullulanase etc. added is more than adequate to completely hydrolyse any starch likely to be present. How...
Thu, 31 Mar, 2016 at 11:42 AM
The method measures inulin and cereal fructans. Product Page (K-FRUC)
Thu, 31 Mar, 2016 at 11:44 AM
What typical absorbance values are obtained for fructan control and fructose control in the Fructan Assay Kit (K-FRUC)?
Fructan Control Flour (29.6%) 0.496 0.498 Fructose Control 1.133 1.135. Product Page (K-FRUC)
Thu, 31 Mar, 2016 at 11:45 AM
Our reproducibility is usually +/- 5 to 7% of the fructan value. Product Page (K-FRUC)
Thu, 31 Mar, 2016 at 11:46 AM
With all our kits we can obtain approx. 5% CV in interlaboratory studies. In-house these values are closer to 3%. Product Page (K-FRUC)
Thu, 31 Mar, 2016 at 11:48 AM
The fructanase in the kit will completely hydrolyse the fructans in plants. These are either inulin type or "branched" type (as in oat or whea...
Thu, 31 Mar, 2016 at 11:49 AM
What is the factor value (F) of 54.5 used in the calculation of the Fructan Assay Kit (K-FRUC) - does it change depending on the fructose content?
The value of 54.5 is the actual amount of fructose standard used. This amount of fructose will give a certain colour in the PAHBAH assay. Thus; F = fac...
Thu, 31 Mar, 2016 at 11:50 AM
Yes. The best enzyme to use for analytical purposes is exo-Inulinase (E-EXOIAN). Product Page (K-FRUC)
Thu, 31 Mar, 2016 at 11:51 AM
Can the sucrase used in the Fructan Assay Kit (K-FRUC) be used to analyse sucrose in extracts of chicory roots?
Yes, the sugar enzyme can be used to measure sucrose. The enzyme is an alpha-glucosidase, so it will also hydrolyse maltose if this is present. P...
Fri, 22 Apr, 2016 at 2:26 PM