The value of 54.5 is the actual amount of fructose standard used. This amount of fructose will give a certain colour in the PAHBAH assay.
Thus; F = factor = [54.5 (micrograms of fructose)] divided by the absorbance value obtained for 54.5 micrograms of fructose in the PAHBAH method.
What is the factor value (F) of 54.5 used in the calculation of the Fructan Assay Kit (K-FRUC) - does it change depending on the fructose content?
Modified on: Wed, 10 Nov, 2021 at 4:49 PM
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