From our experience, the level of beta-amylase/pullulanase etc. added is more than adequate to completely hydrolyse any starch likely to be present. However, if there is a suspicion of incomplete starch hydrolysis, you can check this by running the hydrolysis with the pullulanase/beta-amylase etc. over a range of incubation times and comparing the results.
When measuring fructan in starch containing samples, i.e. maize, is there a possibility of incomplete starch hydrolysis in step B of the Fructan Assay (K-FRUC) ?
Modified on: Thu, 31 Mar, 2016 at 11:42 AM
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