The kit assay will only measure free, non-covalently linked monosaccharides. Oligosaccharides or polysaccharides can be measured after hydrolysis to monosaccharides. Generally acid hydrolysis can be achieved by boiling the oligo/polysaccharide in 1.3 M HCl for 1 h. It is recommended that scientific literature is consulted for information on hydrolysis conditions for the particular oligo/polysaccharide that is being measured.
Can oligosaccharides or polysaccharides be measured using the D-Fructose/D-Glucose Assay Kit (K-FRUGL)?
Modified on: Thu, 31 Mar, 2016 at 11:59 AM
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