The kit assay will only measure free, non-covalently linked monosaccharides.  Oligosaccharides or polysaccharides can be measured after hydrolysis to monosaccharides.  Generally acid hydrolysis can be achieved by boiling the oligo/polysaccharide in 1.3 M HCl for 1 h.  It is recommended that scientific literature is consulted for information on hydrolysis conditions for the particular oligo/polysaccharide that is being measured.

 

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