Protazyme AK Tablets (T-PRAK-200T/T-PRAK-1000T)

What product is recommended to measure activity of proteases such as trypsin or chymotrypsin?
We sell three substrates for the measurement of proteases in general - Protazyme AK Tablets (T-PRAK), Protazyme OL Tablets (T-PROL) and Azo-Casein (S-AZCA...
Tue, 22 Mar, 2016 at 12:13 PM
What is the amount of dye (in milligrams) in the Protazyme Tablets (T-PRAK)?
We add 20% AZCL-collagen/AZCL-casein, i.e. 20 mg/tablet in our Protazyme tablets. Product Page
Tue, 22 Mar, 2016 at 12:13 PM
Is it a requirement to know what type of protease is being measured in order to use Protazyme tablet method (T-PRAK)? Must it be assumed that only fungal proteases are present in bread improvers?
Yes, there is a different regression equation for different proteases.  This is an unfortunate fact for these assays.  Generally however, fungal proteases...
Tue, 22 Mar, 2016 at 12:16 PM
What are the functions of Cysteine and EDTA as components in Buffer B of the Protazyme tablet methods (T-PRAK)?
Cysteine activates thiol proteases such as papain.  EDTA is usually added to bind and remove metal ions which may act as inhibitors. Product Page
Tue, 22 Mar, 2016 at 12:16 PM
Can papain activity be measured using Protazyme tablet tests (T-PRAK) and the activity calculated as Tyr mg/min?
Yes. The calculation for the Protazyme tablet methods give units in micromoles per minute. If you need mg/min, the conversion is: Protease Activity = Pr...
Tue, 22 Mar, 2016 at 12:18 PM
In the Protazyme tablet method (T-PRAK), should the blank be prepared at 40˚C?
Yes. However, when the stopping reagent is added, the tubes should be left at room temperature. Product Page
Tue, 22 Mar, 2016 at 12:18 PM
Which units are used to report the results in the Protazyme tablet methods (T-PRAK)?
With all of our protease assays, we relate back to activity on casein with TCA precipitant and tyrosine as standard (at pH 7). Product Page
Thu, 25 Feb, 2016 at 11:54 AM
Can I use the Protazyme tablet method (T-PRAK) in acidic or alkaline conditions and what type of buffer should I use?
You can use Protazyme AK or OL at a range of pH values. Use acetate buffer in pH range 3.5–5.5; Maleate buffer at 5.5–6.5; Phosphate buffer at 6.2–7.5 and T...
Thu, 10 May, 2018 at 4:58 PM
Is it possible to perform "in feed" determination with the Protazyme tablet methods (T-PRAK)?
It is difficult to determine protease in food samples because these contain a lot of endogenous protein which acts as an alternative substrate. This is a...
Thu, 25 Feb, 2016 at 11:55 AM
Can the Protazyme tablet methods (T-PRAK) be applied to flour?
Yes.   The only limitation on using these methods for flour will be sensitivity.  This you will need to determine yourself. Product Page
Tue, 22 Mar, 2016 at 12:21 PM