This procedure is a suggested guide and may require some optimisation by the user: 

Reaction Mix:

40 μL of pre-heated Soluble Chromogenic Substrate

40 μL sample

Incubation at 40°C for 10 minutes (in flat-bottom 96-well microplate)

Addition of 200 μL precipitation solution -95% (v/v) EtOH, by pipetting vigorously

Centrifugation at 1000g for 10 minutes

Transfer of 150μL supernatant in a new flat-bottom, 96-well microplate

Read at the apprpriate wavelength for the dye being used.

An alternative option may be to process the first step in a 96-well filter plate and filter the supernatant under vacuum into a collection plate.

Product Page (Soluble Chromogenic Substrates)