Can the soluble polysaccharide substrates be used in a microplate format?

Modified on Thu, 23 Sep, 2021 at 4:19 PM

This procedure is a suggested guide and may require some optimisation by the user: 


Reaction Mix:

40 μL of pre-heated Soluble Chromogenic Substrate

40 μL sample


Incubation at 40°C for 10 minutes (in flat-bottom 96-well microplate)


Addition of 200 μL precipitation solution -95% (v/v) EtOH, by pipetting vigorously

Centrifugation at 1000g for 10 minutes


Transfer of 150μL supernatant in a new flat-bottom, 96-well microplate


Read at the apprpriate wavelength for the dye being used.


An alternative option may be to process the first step in a 96-well filter plate and filter the supernatant under vacuum into a collection plate.


Product Page (Soluble Chromogenic Substrates)

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