This procedure is a suggested guide and may require some optimisation by the user:
40 μL of pre-heated Soluble Chromogenic Substrate
40 μL sample
Incubation at 40°C for 10 minutes (in flat-bottom 96-well microplate)
Addition of 200 μL precipitation solution -95% (v/v) EtOH, by pipetting vigorously
Centrifugation at 1000g for 10 minutes
Transfer of 150μL supernatant in a new flat-bottom, 96-well microplate
Read at the apprpriate wavelength for the dye being used.
An alternative option may be to process the first step in a 96-well filter plate and filter the supernatant under vacuum into a collection plate.