β-Glucan Assay Kit (Yeast & Mushroom) (K-YBGL)

In the β-Glucan Assay Kit (Yeast and Mushroom) (K-YBGL) procedure why was the hydrolysis with hydrochloric acid was replaced by hydrolysis with sulphuric acid?
Several acids and enzyme-based methods for the hydrolysis of β-glucan were compared and the best option was the method using H2SO4. For most samples, simi...
Wed, 20 Apr, 2016 at 5:04 PM
What is different between β-Glucan (Yeast & Mushroom) Assay Kit (K-YBGL) and Yeast Beta-Glucan Assay Kit (K-EBHLG)?
β-Glucan (Yeast & Mushroom) Assay Kit (K-YBL) is suitable for the measurement and analysis of 1,3:1,6-beta-glucan, 1,3-β-glucan and α-glucan in yeast...
Mon, 30 May, 2016 at 2:34 PM
Which matrix was used to validated the the β-Glucan Assay Kit (Yeast and Mushroom) (K-YBGL)?
This the β-Glucan Assay Kit (Yeast and Mushroom) has been developed for the measurement of β-glucan in mushroom and mycelial products, i.e. 1,3:1,6-β-gluc...
Wed, 20 Apr, 2016 at 5:05 PM
Can the β-Glucan Assay Kit (Yeast and Mushroom) (K-YBGL) distinguish between β-glucans from mushrooms and those from yeast or molds?
No. β-glucans from mushroom and yeast have the same chemical structure. Fungal and yeast cell walls are composed of ≤ 50% 1,3:1,6-β-glucan. The 1,3:1,6-...
Wed, 20 Apr, 2016 at 5:06 PM
Is it possible to measure specifically 1,3:1,6-β-glucan in a mixed material of mushroom/ yeast and cereal/grains using the β-Glucan Assay Kit (Yeast and Mushroom) (K-YBGL)?
The structures of mushroom and fungal β-glucans are different from the cereal β-glucans. Fungal and yeast cell walls are composed of ≤ 50% 1,3:1,6-β-gluca...
Wed, 20 Apr, 2016 at 5:24 PM
Will samples containing high levels of starch (e.g. mushrooms grown on brown rice, harvested and milled together) cause problems when measuring β-glucan using the β-Glucan Assay Kit (Yeast & Mushroom) (K-YBGL)?
In this method total glucan (plus free glucose and glucose from sucrose) is measured using controlled acid hydrolysis with H2SO4 and the glucose released...
Wed, 20 Apr, 2016 at 5:24 PM
Why is the β-Glucan Assay Kit (Yeast and Mushroom) (K-YBGL) not suitable for measuring yeast β-glucan in the presence of cellulose (1,4-β-D-glucan)?
In this procedure total glucan is measured by hydrolysis with H2SO4, followed by incubation with exo-1,3-β-glucanase/β-glucosidase to ensure complete hyd...
Wed, 20 Apr, 2016 at 5:24 PM
Is it possible to measure soluble and insoluble mushroom β-glucan using the β-Glucan Assay Kit (Yeast and Mushroom) (K-YBGL)?
Yes, this method measures all β-glucans (soluble and insoluble). Product Page (K-YBGL)
Wed, 20 Apr, 2016 at 5:24 PM
Is it possible to check if the β-glucan values for my samples are correct when measured using the β-Glucan Assay Kit (Yeast and Mushroom) (K-YBGL)?
It is highly recommend to analyse the control yeast β-glucan preparation provided with the kit with each set of determinations. If the value received for ...
Wed, 20 Apr, 2016 at 5:23 PM
What is the repeatability of the β-Glucan Assay Kit (Yeast and Mushroom) (K-YBGL)?
Standard errors of ± 5% are achieved routinely within our laboratory. However it is up to each individual customer to decide if their results are within a...
Wed, 20 Apr, 2016 at 5:23 PM