In this method total glucan (plus free glucose and glucose from sucrose) is measured using controlled acid hydrolysis with H2SO4 and the glucose released is measured using glucose oxidase/peroxidase reagent. α-Glucan (starch/glycogen) plus free glucose and glucose from sucrose are measured after hydrolysis of starch/glycogen to glucose with glucoamylase and sucrose to glucose plus fructose with invertase and the glucose measured with GOPOD reagent. β-Glucan is determined by the difference. For high starch and low β-glucan samples the β-glucan values can occasionally appear to show a negative value. This can be explained considering the standard deviation of the method.

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