In this method total glucan (plus free glucose and glucose from sucrose) is measured using controlled acid hydrolysis with H2SO4 and the glucose released is measured using glucose oxidase/peroxidase reagent. α-Glucan (starch/glycogen) plus free glucose and glucose from sucrose are measured after hydrolysis of starch/glycogen to glucose with glucoamylase and sucrose to glucose plus fructose with invertase and the glucose measured with GOPOD reagent. β-Glucan is determined by the difference. For high starch and low β-glucan samples the β-glucan values can occasionally appear to show a negative value. This can be explained considering the standard deviation of the method.
Will samples containing high levels of starch (e.g. mushrooms grown on brown rice, harvested and milled together) cause problems when measuring β-glucan using the β-Glucan Assay Kit (Yeast & Mushroom) (K-YBGL)?
Modified on: Wed, 20 Apr, 2016 at 5:24 PM
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