The glucose measurement (incubation with GOPOD Reagent) is linear between 4 and 100 micrograms of glucose per assay. We recommend that the smallest differentiating absorbance ΔA should be at least 0.04 with GOPOD Reaction. This is an equivalent of 3.6% of β-glucan in a powder sample, assuming there is no α-glucan present. To increase the limit of detection, you can decrease the volume during adjusting after the acid hydrolysis, but you need to ensure that the pH of the sample solution is close to 5.0, before proceeding to the next step.