In this procedure total glucan is measured by hydrolysis with H2SO4, followed by incubation with exo-1,3-β-glucanase/β-glucosidase to ensure complete hydrolysis of laminari-oligosaccharides to glucose. α-Glucan is measured specifically using a starch assay procedure and β-glucan is determined by difference. Under the assay conditions (acid hydrolysis) beta-glucans present in the sample will be hydrolysed, including 1,3, 1,3:1,6, 1,3:1,4-β-glucan and most of  1,4-β-D-glucan (cellulose), which will lead to overestimation of 1,3:1,6-β-glucan.

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