This temperature range is recommended in order to ensure that the ethanol does not boil out of the top of the tube. A boiling water bath can be used at this step and a bigger tube should be used. The higher temperature will have no effect on the sugars.
Is it possible to carry out the enzyme inactivation step in K-RAFGL in a boiling water bath instead of at 84-88C?
Modified on: Fri, 30 Oct, 2020 at 7:42 AM
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