The D-Mannose/D-Fructose/D-Glucose assay is linear over the range of 4 to 80 μg of D-glucose, D-fructose or D-mannose per assay. Using a 0.1 mL assay volume of the supplied D-Glucose, D-fructose plus D-mannose standard solution at 0.10 mg/mL of each sugar will correspond to 10 µg of D-glucose, D-fructose and D-mannose in the assay.
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