Mannan (Ivory Nut) (P-MANIV)

What is the degree of polymerisation of Mannan (P-MANIV) and does it contain any small soluble oligosaccharides?
We do not have accurate degree of polymerisation data for mannan from ivory nut but believe the value to be in the range of 15-20.  It does not contain s...
Tue, 22 Mar, 2016 at 2:55 PM
How have you extracted the mannan (P-MANIV) from Ivory Nut?
The ivory meal is ground and defatted (Chloroform/methanol) and dried.  It is then cooked at 80˚C in 1% HCL for 1 h.  The recovered residue is then extract...
Tue, 22 Mar, 2016 at 2:56 PM
If I want to degrade mannan by Nelson / Somogyi method in order to determine the amount of mannose, which product is appropriate to this assay and is there a better method than hydrolysis?
Mannan is best degraded by hydrolysis with 2 M trifluoracetic acid at 120°C for 60 min (in an oil bath). We normally hydrolyse 10 mg sample in 2 mL of th...
Tue, 22 Mar, 2016 at 2:57 PM