If I want to degrade mannan by Nelson / Somogyi method in order to determine the amount of mannose, which product is appropriate to this assay and is there a better method than hydrolysis?

Modified on Wed, 5 May, 2021 at 3:50 PM

Mannan is best degraded by hydrolysis with 2 M trifluoracetic acid at 120°C for 60 min (in an oil bath). We normally hydrolyse 10 mg sample in 2 mL of the acid. Mannan will be completely hydrolysed by this treatment.