The unit of activity for the pullulanase assay using Red Pullulan is:
 One unit of activity is the amount of enzyme required to split one micromole of 1,6-linkages per minute under the defined assay conditions. The enzyme is standardised using pullulan (reduced) as substrate, with measurement of increase in reducing sugar level using the Nelson/Somogyi procedure with glucose as standard.

Product Page (S-RPUL)