The unit of activity for the pullulanase assay using Red Pullulan is:
One unit of activity is the amount of enzyme required to split one micromole of 1,6-linkages per minute under the defined assay conditions. The enzyme is standardised using pullulan (reduced) as substrate, with measurement of increase in reducing sugar level using the Nelson/Somogyi procedure with glucose as standard.
What is the I’d like to know the International Unit definition when using your Red-Pullulan (S-RPUL) assay to determine debranching enzyme activity?
Modified on: Thu, 23 Sep, 2021 at 4:03 PM
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