Azo-Barley Glucan (S-ABG100)
Are Azo-Barley Glucan (S-ABG100) & Azo-Xylan the best substrates to analyse Trichoderma reseei xylanase and beta-glucanase in animal feed?
The best substrate for beta-glucanase in animal feed is Azo-Barley Glucan; however, beta-Glucazyme tablets also work well. Xylanase is best measured with ...
Wed, 23 Mar, 2016 at 12:44 PM
Do you have a typical figure for the ratio of (1-3) to (1-4) linkages in the beta-glucan you use in the Azo-Barley beta-Glucan?
The ratio of 1,3 to 1,4 linkages in the beta-glucan we use to make Azo-Barley beta-glucan (i.e. Barley beta-glucan) is ~ 32:68. This ratio is constant fo...
Wed, 23 Mar, 2016 at 12:46 PM
What is the concentration of Azo-Barley glucan as supplied in liquid form? Also, can this substrate be used to make an agar plate pH 7.0–7.2?
Azo-Barley Glucan is supplied at 1% w/v. This can be used in an agar plate. It is provided unbuffered but with 0.2% sodium azide as a preservative. Per...
Wed, 23 Mar, 2016 at 12:47 PM
We use the dye Remazol Brilliant Blue. Product Page (S-ABG100)
Fri, 21 Oct, 2016 at 2:47 PM
Can Azo-Barley Glucan (S-ABG100) be used to assay at 65˚C to replicate mash conditions? I understand that the standard curve given in the method will no longer apply but I am mostly interested in comparative results.
You can use Azo-Barley Glucan at 65˚C. There will be no problems with stability at 65˚C and pH 5.5. The pH of the buffer could simply be adjusted with 1...
Wed, 23 Mar, 2016 at 12:49 PM
We are using your Azo-Barley Glucan (S-ABG100) solution as substrate for bacterial beta-glucanase screening. The original method uses Zn-acetate and methoxyethanol as precipitant. All other Azo-substrates use ethanol as precipitant. Why?
We use the Zn acetate in the methoxyethanol to help precipitate all the protein in malt extracts (i.e. to prevent haze). However, we have recently devel...
Wed, 23 Mar, 2016 at 12:51 PM