We use the Zn acetate in the methoxyethanol to help precipitate all the protein in malt extracts (i.e. to prevent haze). However, we have recently developed an alternative precipitant [Industrial Methylated Spirits (IMS)/Zinc acetate] for this assay. The details of this precipitant is given in the updated version of the Malt Beta Glucanase Kit booklet.
We are using your Azo-Barley Glucan (S-ABG100) solution as substrate for bacterial beta-glucanase screening. The original method uses Zn-acetate and methoxyethanol as precipitant. All other Azo-substrates use ethanol as precipitant. Why?
Modified on: Wed, 23 Mar, 2016 at 12:51 PM
Did you find it helpful?Send feedback
Sorry we couldn't be helpful. Help us improve this article with your feedback.