α-Amylase Assay Kit (Ceralpha Method) (K-CERA)

Is the α-Amylase Assay Kit (Ceralpha Method) (K-CERA) suitable for measuring porcine pancreatic alpha-amylase?
The Ceralpha method is excellent for all alpha-amylases and will work fine for porcine pancreas alpha-amylase at pH 6.9. Product Page (K-CERA)
Tue, 29 Mar, 2016 at 2:13 PM
We would like to know where the coefficient of 18.1 (EmM of p-nitrophenol) comes from? What does it mean?
This is the extinction coefficient, i.e. absorbance of a 1 mM solution of p-nitrophenol in a 1 cm light path at 400 nm. Product Page (K-CERA)
Tue, 29 Mar, 2016 at 2:14 PM
Any recommendations on using your α-Amylase Assay Kit (Ceralpha Method) (K-CERA) assay at a low pH (pH 3-4)?
Cereal alpha-amylase assay reagent cannot be used at pH 3-4.  However, you can perform a similar assay using blocked p-nitophenol maltoheptaoside (BPNPG7...
Tue, 29 Mar, 2016 at 2:22 PM
Can the α-Amylase Assay Kit (Ceralpha Method) (K-CERA) distinguish between different forms of alpha-amylase?
The Ceralpha Kit does not distinguish between the different forms of alpha amylase, however some idea of relative levels of fungal and bacterial alpha-amy...
Tue, 29 Mar, 2016 at 2:22 PM
To measure alpha-amylase in wheat grains using the α-Amylase Assay Kit (Ceralpha Method) (K-CERA) to test for the presence of alpha-amylase, do wheat grains have to be milled or can a blender be used?
Cereal grains do need to be milled to allow effective extraction of alpha-amylase (and other constituents). We recommend milling to pass a 0.5 mm screen....
Wed, 30 Mar, 2016 at 9:50 AM
Can the α-Amylase Assay Kit (Ceralpha Method) (K-CERA) reagent be used at high temperatures?
The Ceralpha method is routinely run at 40˚C, but it can be used at temperatures up to 60˚C. If it is run at higher temperatures (up to 60˚C) then higher...
Wed, 30 Mar, 2016 at 9:51 AM
Can α-Amylase Assay Kit (Ceralpha Method) (K-CERA) determine the alpha-amylase activity in a protease preparation?
The standard method should be fine. You can best check this by performing a time course hydrolysis of the alpha-amylase. This should be linear if the a...
Wed, 30 Mar, 2016 at 9:52 AM
Can the substrate used in the α-Amylase Assay Kit (Ceralpha Method) (K-CERA) only be hydrolysed by alpha-amylase?
Yes, only alpha-amylase can cleave the internal glycosidic bonds of the Ceralpha substrate Product Page (K-CERA)
Wed, 30 Mar, 2016 at 9:54 AM
After alpha-amylase cleaves the internal glycosidic linkage of the Ceralpha substrate can beta-amylase or glucoamylase accelerate the hydrolysis reaction of the released p-nitrophenyl maltosaccharide?
When alpha-amylase cleaves the glycosidic linkage in the blocked substrate, any other enzymes in the extract do not accelerate the hydrolysis to give fre...
Wed, 30 Mar, 2016 at 9:56 AM
Can Rice alpha-amylase be analysed by the α-Amylase Assay Kit (Ceralpha Method) (K-CERA)?
Rice alpha-amylase can be assayed with Ceralpha kit. Alpha-glucosidase in the rice extract will not interfere. Product Page (K-CERA)
Wed, 30 Mar, 2016 at 9:57 AM