α-Amylase Assay Kit (Ceralpha Method) (K-CERA)
The
Ceralpha method is excellent for all alpha-amylases and will work fine for
porcine pancreas alpha-amylase at pH 6.9.
Product Page (K-CERA)
Tue, 29 Mar, 2016 at 2:13 PM
This is
the extinction coefficient, i.e. absorbance of a 1 mM solution
of p-nitrophenol in a 1 cm light path at 400 nm.
Product Page (K-CERA)
Tue, 29 Mar, 2016 at 2:14 PM
Cereal
alpha-amylase assay reagent cannot be used at pH 3-4. However, you can
perform a similar assay using blocked p-nitophenol maltoheptaoside
(BPNPG7...
Tue, 29 Mar, 2016 at 2:22 PM
The
Ceralpha Kit does not distinguish between the different forms of alpha amylase,
however some idea of relative levels of fungal and bacterial alpha-amy...
Tue, 29 Mar, 2016 at 2:22 PM
Cereal
grains do need to be milled to allow effective extraction of alpha-amylase (and
other constituents). We recommend milling to pass a 0.5 mm screen....
Wed, 30 Mar, 2016 at 9:50 AM
The
Ceralpha method is routinely run at 40˚C, but it can be used at temperatures up
to 60˚C. If it is run at higher temperatures (up to 60˚C) then higher...
Wed, 30 Mar, 2016 at 9:51 AM
The
standard method should be fine. You can best check this by performing a
time course hydrolysis of the alpha-amylase. This should be linear if the
a...
Wed, 30 Mar, 2016 at 9:52 AM
Yes, only
alpha-amylase can cleave the internal glycosidic bonds of the Ceralpha substrate
Product Page (K-CERA)
Wed, 30 Mar, 2016 at 9:54 AM
When
alpha-amylase cleaves the glycosidic linkage in the blocked substrate, any
other enzymes in the extract do not accelerate the hydrolysis to give
fre...
Wed, 30 Mar, 2016 at 9:56 AM
Rice
alpha-amylase can be assayed with Ceralpha kit. Alpha-glucosidase in the
rice extract will not interfere.
Product Page (K-CERA)
Wed, 30 Mar, 2016 at 9:57 AM
