To measure alpha-amylase on glass beads we recommend the use of the Ceralpha method (K-CERA) or the α-Amylase SD Assay Kit (High Sensitivity Method) (K-AMYLSD). Both kits are complete and extremely easy to use. Perhaps to assay, you may freeze dry a small sample of the beads and then weigh an appropriate amount (say 5 mg) into a test tube. Add 0.2 mL of buffer pH 5-6 and 0.2 mL Ceralpha reagent. Incubate at 40˚C for 10 min.
I have immobilised alpha-amylase onto glass bead and now I want to test the activity, can the Ceralpha (K-CERA) or Amylase SD (K-AMYLSD) methods be used for this?
Modified on: Wed, 30 Mar, 2016 at 10:07 AM
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