The Ceralpha method can be used for oats. The only likely problem is the high viscosity of the extract. If the extract looks very viscous, double the ratio of extraction buffer to flour. Allow for this in the calculations. If the absorbances are low then increase the incubation time to say 30 minutes. Then allow for this increased time of incubation in the calculations.
Can the α-Amylase Assay Kit (Ceralpha Method) (K-CERA) be used to determine alpha-amylase from oat flour extracts?
Modified on: Wed, 30 Mar, 2016 at 9:59 AM
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