Glucose Determination Reagent (R-GLC4)

How long is the GOPOD reagent (K-GLUC / R-GLC4) stable for when stored at 4˚C?
This reagent, after preparation, can be stored for 3 months at 4˚C. It is quite common for the GOPOD reagent to change from a pale yellow colour when fres...
Mon, 4 Apr, 2016 at 12:48 PM
Can the absorbance for the GOPOD assay (R-GLC4 / K-GLUC) be measured at alternative wavelengths if 510 nm is not available?
Yes. The most important thing is that all solutions including the glucose standard control must be read at the same wavelength.If the absorbance readings f...
Mon, 4 Apr, 2016 at 12:48 PM
What might be the cause of very low absorbance readings for glucose controls and samples in the GOPOD assay procedure (K-GLUC / R-GLC4)?
Always ensure that both components of the GOPOD reagent, i.e. Glucose Reagent Buffer and Glucose Determination Reagent, are well mixed, as per the data book...
Thu, 10 May, 2018 at 3:49 PM
Is the absorbance reading for the GOPOD assay (K-GLUC / R-GLC4) affected by any components likely to be present in samples?
The colour reaction may be affected by high salt concentrations above 0.2 M and possibly by ascorbate. To test for possible effects on colour developmen...
Mon, 4 Apr, 2016 at 12:54 PM
What kind of precautions should be taken for handling the GOPOD reagent (K-GLUC / R-GLC4) containing sodium azide?
Use disposable gloves when handling the concentrate. After dilution, the concentration of azide is very low (0.02%) but the buffer should still be handl...
Mon, 4 Apr, 2016 at 12:54 PM
What is the linear range of the Glucose Determination Reagent (R-GLC4)?
The test is set up to measure between 4 and 100 μg per assay (i.e. 0.1 mL of 0.04 to 1.0 mg/mL of glucose). If the glucose concentration in your sample is...
Fri, 22 Apr, 2016 at 12:08 PM
What is the lower limit of detection of glucose measurement using the Glucose Determination Reagent (R-GLC4)?
We recommend that the smallest differentiating absorbance ΔA should be at least 0.04. This is the equivalent of approximately 4 μg of glucose in 0.1 mL in...
Fri, 22 Apr, 2016 at 12:11 PM
Is it possible to increase the sensitivity of the Glucose Determination Reagent (R-GLC4)?
To increase the sensitivity of the GOPOD assay you can reduce the volume of GOPOD Reagent used, e.g. decreasing the volume to 1 mL will increase the sensi...
Fri, 22 Apr, 2016 at 12:11 PM
Is it possible to scale down the volumes of reagents used in the Glucose Determination Reagent (R-GLC4)?
If you wish to scale down the GOPOD assay you should proportionally decrease all volumes used in the assay: samples, glucose standard and GOPOD Reagent vo...
Fri, 22 Apr, 2016 at 12:11 PM
Is it possible to read absorbances for GOPOD Reagent reaction (R-GLC4) at a wavelength other than 510 nm?
While we recommend reading the absorbance at 510 nm you can use different wavelength if that is your preference. However please note this may give a large...
Fri, 22 Apr, 2016 at 12:12 PM