D-Glucose (GOPOD Format) (K-GLUC)
The test is best run at pH 7.4. The reagent is buffered at this pH. Using different pH values will affect results, i.e. it may take longer to reach this end...
Wed, 20 Apr, 2016 at 4:43 PM
The test is
set up to measure between 4 and 100 micrograms per assay (i.e. 0.1 mL of 0.04
to 1.0 mg/mL of glucose). If the glucose
concentration in your ...
Wed, 20 Apr, 2016 at 4:43 PM
The
reaction is complete after approx. 15 min, and the colour is stable at
temperatures of 20-50˚C for at least an extra hour.
Product Page (K-GLU...
Wed, 20 Apr, 2016 at 4:44 PM
~ 1 microg per total sample volume added to the assay.
1
microgram gives an absorbance of 0.01 if 3 mL of GOPOD are used. If 1 mL
of GOPOD is use...
Wed, 20 Apr, 2016 at 4:44 PM
The
temperature is just for the incubations. It is an end-point assay, so the
spectrophotometer does not need to be temperature controlled.
Produ...
Wed, 20 Apr, 2016 at 4:44 PM
The kit enzymes can be stored at 0-5°C for up to 12 months.
Product Page (K-GLUC)
Wed, 20 Apr, 2016 at 4:44 PM
The kit
assay will only measure free, non-covalently linked monosaccharides. Oligosaccharides or polysaccharides can be measured after hydrolysis to monos...
Mon, 4 Apr, 2016 at 12:42 PM
We recommend
that the smallest differentiating absorbance ΔA should be at least 0.04. This
is the equivalent of approximately 4 micrograms of glucose in 0...
Wed, 20 Apr, 2016 at 4:45 PM
To increase the sensitivity of the GOPOD assay you can
reduce the volume of GOPOD Reagent used, e.g. decreasing the volume to 1 mL
will increase the sensi...
Wed, 20 Apr, 2016 at 4:45 PM
If you wish to scale down the
GOPOD assay you should proportionally decrease all volumes used in the assay:
samples, glucose standard and GOPOD Reagent vo...
Wed, 20 Apr, 2016 at 4:36 PM