D-Glucose (GOPOD Format) (K-GLUC)

Will results be incorrect if it is outside a certain pH range from the pH optimum of glucose oxidase and peroxidase in the D-Glucose Assay Kit (GOPOD Format) (K-GLUC)?
The test is best run at pH 7.4. The reagent is buffered at this pH. Using different pH values will affect results, i.e. it may take longer to reach this end...
Wed, 20 Apr, 2016 at 4:43 PM
What is the linear range of the D-Glucose Assay Kit (GOPOD Format) (K-GLUC)?
The test is set up to measure between 4 and 100 micrograms per assay (i.e. 0.1 mL of 0.04 to 1.0 mg/mL of glucose). If the glucose concentration in your ...
Wed, 20 Apr, 2016 at 4:43 PM
In the D-Glucose Assay Kit (GOPOD Format) (K-GLUC Assay) is the colour stable at room temperature? Does the reaction continue if not read within 20 minutes?
The reaction is complete after approx. 15 min, and the colour is stable at temperatures of 20-50˚C for at least an extra hour. Product Page (K-GLU...
Wed, 20 Apr, 2016 at 4:44 PM
What is the lower limit of detection of D-Glucose Assay Kit (GOPOD Format) (K-GLUC)?
~ 1 microg per total sample volume added to the assay. 1 microgram gives an absorbance of 0.01 if 3 mL of GOPOD are used. If 1 mL of GOPOD is use...
Wed, 20 Apr, 2016 at 4:44 PM
The temperature stated for the D-Glucose Assay Kit (GOPOD Format) (K-GLUC) procedure is 40 or 50˚C - is this for incubation and / or reading the results on the spectrophotometer?
The temperature is just for the incubations. It is an end-point assay, so the spectrophotometer does not need to be temperature controlled. Produ...
Wed, 20 Apr, 2016 at 4:44 PM
What is the shelf-life (period of stability) of the D-Glucose Assay Kit (GOPOD Format) (K-GLUC) components when stored at 0-5°C?
The kit enzymes can be stored at 0-5°C for up to 12 months. Product Page (K-GLUC)
Wed, 20 Apr, 2016 at 4:44 PM
Can oligosaccharides or polysaccharides be measured using the D-Glucose Assay Kit (GOPOD Format) (K-GLUC)?
The kit assay will only measure free, non-covalently linked monosaccharides.  Oligosaccharides or polysaccharides can be measured after hydrolysis to monos...
Mon, 4 Apr, 2016 at 12:42 PM
What is the lower limit of detection of glucose measurement using the D-Glucose Assay Kit (GOPOD Format) (K-GLUC)?
We recommend that the smallest differentiating absorbance ΔA should be at least 0.04. This is the equivalent of approximately 4 micrograms of glucose in 0...
Wed, 20 Apr, 2016 at 4:45 PM
Is it possible to increase the sensitivity of the D-Glucose Assay Kit (GOPOD Format) (K-GLUC)?
To increase the sensitivity of the GOPOD assay you can reduce the volume of GOPOD Reagent used, e.g. decreasing the volume to 1 mL will increase the sensi...
Wed, 20 Apr, 2016 at 4:45 PM
Is it possible to scale down the volumes of reagents used in the D-Glucose Assay Kit (GOPOD Format) (K-GLUC)?
If you wish to scale down the GOPOD assay you should proportionally decrease all volumes used in the assay: samples, glucose standard and GOPOD Reagent vo...
Wed, 20 Apr, 2016 at 4:36 PM