The kit assay will only measure free, non-covalently linked monosaccharides. Oligosaccharides or polysaccharides can be measured after hydrolysis to monosaccharides. Generally acid hydrolysis can be achieved by boiling the oligo/polysaccharide in 1.3 M HCl for 1 h. It is recommended that scientific literature is consulted for information on hydrolysis conditions for the particular oligo/polysaccharide that is being measured.
Can oligosaccharides or polysaccharides be measured using the D-Glucose Assay Kit (GOPOD Format) (K-GLUC)?
Modified on: Mon, 4 Apr, 2016 at 12:42 PM
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