Primary Amino Nitrogen Assay Kit (PANOPA) (K-PANOPA)

When using the Primary Amino Nitrogen Assay Kit (K-PANOPA) for quantitative analysis what level of accuracy and repeatability can be expected?
The Primary Amino Nitrogen Assay Kit is extremely accurate – at Megazyme the quality control criteria for accuracy and repeatability is to be within 2% of ...
Mon, 18 Apr, 2016 at 11:42 AM
What kits are recommended to measure yeast assimilable nitrogen (YAN) during wine production?
YAN is made up of nitrogen from primary amino nitrogen, ammonia plus urea and all of these can be measured using the Primary Amino Nitrogen Assay Kit (K-PAN...
Thu, 10 May, 2018 at 3:11 PM
What is the detection limit of the Primary Amino Nitrogen Assay Kit (K-PANOPA)?
The detection limit of the Primary Amino Nitrogen Assay Kit is 2.59 mg/L, which is derived from an absorbance difference of 0.020 with the maximum sample ...
Mon, 18 Apr, 2016 at 11:43 AM
What is the linearity of the Primary Amino Nitrogen Assay Kit (K-PANOPA)?
The Primary Amino Nitrogen assay is linear over the range 0.2 to 10 µg of amino nitrogen per assay.  Using a 0.05 mL assay volume of the supplied isoleucin...
Mon, 18 Apr, 2016 at 11:44 AM
When using the Primary Amino Nitrogen Assay Kit (K-PANOPA) with the MegaQuant™ Wave Spectrophotometer (D-MQWAVE), how can I minimise the exposure to light?
The use of foil to cover the tubes during the incubations and reading steps works very well to eliminate the exposure to light in this application. Prod...
Thu, 10 May, 2018 at 3:11 PM
Can Bottle 2 (OPA) of the Primary Amino Nitrogen Assay Kit (K-PANOPA) be diluted with water to increase the volume of R2 that is used in the auto-analyser format?
Yes. The OPA solution can be diluted as described for the auto-analyser fomrat in the K-PANOPA kit data booklet. Product Page (K-PANOPA)
Thu, 10 May, 2018 at 3:12 PM
Why might the primary amine be undetectable after a 10 min reaction but detectable after 15 min using the Primary Amino Nitrogen Assay Kit (K-PANOPA)? The test samples contain ammonium ions and primary amine.
Interference can be caused by ammonium ions during the first 10 minutes of the reaction generating a false positive reading so the reaction must be allowe...
Mon, 18 Apr, 2016 at 11:57 AM
Can the K-PANOPA kit be used to measure Free Amino Nitrogen (FAN)?
Yes the K-PANOPA kit can be used to measure free amino nitrogen (FAN). A traditional method to measure nitrogen is the formol method and correlation exi...
Thu, 10 May, 2018 at 3:12 PM